Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Macaca fuscata | |
hydroxylamine | - |
Rattus norvegicus | |
Isonicotinic hydrazide | - |
Macaca fuscata | |
Isonicotinic hydrazide | - |
Rattus norvegicus | |
KCN | - |
Macaca fuscata | |
KCN | - |
Rattus norvegicus | |
Semicarbazide | - |
Macaca fuscata | |
Semicarbazide | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
glyoxylate | - |
Macaca fuscata | |
0.5 | - |
glyoxylate | - |
Rattus norvegicus | |
1.2 | - |
L-kynurenine | - |
Rattus norvegicus | |
1.5 | - |
L-kynurenine | - |
Macaca fuscata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | - |
Macaca fuscata | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Rattus norvegicus |
40000 | - |
2 * 40000, SDS-PAGE | Macaca fuscata |
80000 | - |
gel filtration | Rattus norvegicus |
80000 | - |
gel filtration | Macaca fuscata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + glyoxylate | Rattus norvegicus | glucagon inducible enzyme | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | Macaca fuscata | glucagon inducible enzyme | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | Rattus norvegicus Donryu | glucagon inducible enzyme | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca fuscata | - |
Blyth monkey | - |
Rattus norvegicus | - |
Donryu strain | - |
Rattus norvegicus Donryu | - |
Donryu strain | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Macaca fuscata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Macaca fuscata | - |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, at least 4 weeks | Macaca fuscata |
-20°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, at least 7 weeks | Rattus norvegicus |
0-4°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, 2 weeks | Macaca fuscata |
0-4°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal phosphate, 5 mM mercaptoethanol, 20% glycerol, 3 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxytryptophan + glyoxylate | - |
Rattus norvegicus | 3-(5-hydroxyindole)-2-oxopropanoate + glycine | - |
? | |
5-hydroxytryptophan + glyoxylate | - |
Macaca fuscata | 3-(5-hydroxyindole)-2-oxopropanoate + glycine | - |
? | |
histidine + glyoxylate | - |
Rattus norvegicus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine | - |
? | |
histidine + glyoxylate | - |
Macaca fuscata | 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine | - |
? | |
L-kynurenine + 2-oxoglutarate | - |
Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate | - |
? | |
L-kynurenine + 4-methylsulfanyl-2-oxobutanoate | - |
Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine | - |
? | |
L-kynurenine + 4-methylsulfanyl-2-oxobutanoate | - |
Macaca fuscata | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine | - |
? | |
L-kynurenine + 4-methylsulfanyl-2-oxobutanoate | - |
Rattus norvegicus Donryu | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine | - |
? | |
L-kynurenine + glyoxylate | - |
Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | - |
Macaca fuscata | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | glucagon inducible enzyme | Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | glucagon inducible enzyme | Macaca fuscata | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | - |
Rattus norvegicus Donryu | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + glyoxylate | glucagon inducible enzyme | Rattus norvegicus Donryu | 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine | - |
? | |
L-kynurenine + hydroxypyruvate | - |
Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine | - |
? | |
L-kynurenine + hydroxypyruvate | - |
Macaca fuscata | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine | - |
? | |
L-kynurenine + hydroxypyruvate | - |
Rattus norvegicus Donryu | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine | - |
? | |
L-kynurenine + pyruvate | - |
Rattus norvegicus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine | - |
? | |
L-kynurenine + pyruvate | - |
Macaca fuscata | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine | - |
? | |
L-kynurenine + pyruvate | - |
Rattus norvegicus Donryu | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine | - |
? | |
phenylalanine + glyoxylate | - |
Rattus norvegicus | phenylpyruvate + glycine | - |
? | |
phenylalanine + glyoxylate | - |
Macaca fuscata | phenylpyruvate + glycine | - |
? | |
tryptophan + glyoxylate | - |
Rattus norvegicus | 3-indole-2-oxopropanoate + glycine | - |
? | |
tryptophan + glyoxylate | - |
Macaca fuscata | 3-indole-2-oxopropanoate + glycine | - |
? | |
tyrosine + glyoxylate | - |
Rattus norvegicus | 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine | - |
? | |
tyrosine + glyoxylate | - |
Macaca fuscata | 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 40000, SDS-PAGE | Macaca fuscata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Macaca fuscata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
t1/2: 25 min | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, tightly bound | Rattus norvegicus | |
pyridoxal 5'-phosphate | requirement, tightly bound | Macaca fuscata |