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Literature summary for 2.6.1.62 extracted from

  • Dey, S.; Lane, J.M.; Lee, R.E.; Rubin, E.J.; Sacchettini, J.C.
    Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase (2010), Biochemistry, 49, 6746-6760.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.62

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.2 A resolution, by molecular replacement, and superimposition of the structures bound either to the S-adenosyl-L-methionine analog sinefungin or to 7-oxo-8-aminopelargonic acid. Comparison to structure of the Bacillus subtilis enzyme, EC 2.5.1.105 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 S-adenosyl-L-methionine pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis
0.7
-
 sinefungin pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQ80
-
-
Mycobacterium tuberculosis CDC 1551 P9WQ80
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
S-adenosyl-L-methionine + 8-amino-7-oxononanoate
-
 Mycobacterium tuberculosis CDC 1551 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
S-adenosyl-L-methionine + sinefungin
-
 Mycobacterium tuberculosis S-adenosyl-4-methylthio-2-oxobutanoate + ?
-
 ?
S-adenosyl-L-methionine + sinefungin
-
 Mycobacterium tuberculosis CDC 1551 S-adenosyl-4-methylthio-2-oxobutanoate + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 sinefungin pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis
0.012
-
 S-adenosyl-L-methionine pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.013
-
 sinefungin pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis
0.03
-
 S-adenosyl-L-methionine pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis