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Literature summary for 2.6.1.58 extracted from
- Species distribution and properties of hepatic phenylalanine (histidine):pyruvate aminotransferase (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 59-67.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus |  |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing, loss of most of activity | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| histidine | 10 mM: 56% inhibition with L-phenylalanine and pyruvate as substrates | Mus musculus | |
| hydroxylamine | 0.5 mM: 63% inhibition | Mus musculus | |
| Isonicotinic acid hydrazide | 2 mM: 27% inhibition | Mus musculus | |
| KCN | 1 mM: 22% inhibition | Mus musculus | |
| L-methionine | 10 mM: competitive inhibitor | Mus musculus | |
| p-chloromercuribenzoate | 0.25 mM: 48% inhibition, reactivation by addition of 2-mercaptoethanol | Mus musculus | |
| Semicarbazide | 1 mM: 12% inhibition | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.9 | - |
histidine | cosubstrate pyruvate, pH 8.9, 37°C | Mus musculus | |
| 21 | - |
pyruvate | cosubstrate L-histidine, pH 8.9, 37°C | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | mitochondrial fraction: 70% of enzymatic activity, cytosolic fraction: 12% | Mus musculus | 5829 | - |
| mitochondrion | mitochondrial fraction: 70% of enzymatic activity, cytosolic fraction: 12% | Mus musculus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | 81000 | gel filtration | Mus musculus |
| 75000 | 85000 | sucrose density gradient centrifugation | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine + pyruvate | Mus musculus | - |
phenylpyruvate + L-alanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
| Cavia porcellus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
injection of glucagon elevates activity | - |
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
injection of glucagon elevates activity | - |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, isoelectric focusing of the purified enzyme results in detection of 4 forms with different pI, all forms are nearly identical with respect to physical and enzymatic properties, all forms are responsive to glucagon | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.8 | - |
L-histidine with pyruvate, pH 8.9, 37°C | Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme in 0.05 mM potassium phosphate buffer, pH 7.5, rapid inactivation | Mus musculus |
| 4°C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, stable for at least 2 weeks | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + phenylpyruvate | - |
Mus musculus | 5-amino-2,5-dioxopentanoic acid + L-phenylalanine | - |
? | |
| L-histidine + pyruvate | about 50% of activity compared to L-phenylalanine | Mus musculus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
? | |
| L-methionine + phenylpyruvate | - |
Mus musculus | 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
? | |
| L-phenylalanine + pyruvate | - |
Mus musculus | phenylpyruvate + L-alanine | - |
? | |
| L-phenylalanine + pyruvate | highest activity | Mus musculus | phenylpyruvate + L-alanine | - |
? | |
| L-serine + phenylpyruvate | - |
Mus musculus | 3-hydroxy-2-oxopropanoate + L-phenylalanine | - |
? | |
| L-tyrosine + pyruvate | about 27% of activity compared to L-phenylalanine | Mus musculus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine | - |
? | |
| additional information | oxaloacetate and hydroxypyruvate may replace pyruvate as amino acceptor at lower rates, little or no activity with 2-oxoglutarate | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000-42000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
| 37 | - |
assay at | Cavia porcellus |
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Sus scrofa |
| 37 | - |
assay at | Oryctolagus cuniculus |
| 37 | - |
assay at | Canis lupus familiaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
1 h, in 15 mM potassium phosphate buffer, pH 7.5: stable | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
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