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Literature summary for 2.6.1.57 extracted from

  • Noguchi, T.
    Aromatic-amino-acid aminotransferase from small intestine (1987), Methods Enzymol., 142, 267-273.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
2-oxoglutarate cosubstrate phenylalanine Rattus norvegicus
7.7
-
L-phenylalanine pH 8.0. 37°C Rattus norvegicus
10
-
L-tryptophan
-
Rattus norvegicus
11.8
-
L-tyrosine pH 8.0. 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, SDS-PAGE Rattus norvegicus
100000
-
gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
vitamin B6-deficient strain
-

Purification (Commentary)

Purification (Comment) Organism
vitamin B6-deficient strain, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephacryl s-200, hydroxylapatite Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
small intestine mucosa Rattus norvegicus
-
small intestine not in muscle phase Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.4
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, at least 3 weeks, no loss of activity Rattus norvegicus
0-4°C, 2 weeks, little loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxytryptophan + 2-oxoglutarate 19.5% of the activity with phenylalanine Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate best amino acid substrate, specific for 2-oxoglutarate, little or no activity with glyoxylate, pyruvate, 2-oxo-4-methylthiobutyrate and alpha-ketoadipate Rattus norvegicus phenylpyruvate + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate 39.7% of activity with phenylalanine Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate 23.7% of activity with phenylalanine Rattus norvegicus p-hydroxyphenylpyruvate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
approx. 30% and 27% loss of activity after 10 min with L-tyrosine and L-phenylalanine, respectively, as subtrates Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
with phenylalanine Rattus norvegicus