Crystallization (Comment) | Organism |
---|---|
ammonium sulfate at pH 7.0-7.6, crystals appear after 2-3 days | Achromobacter superficialis |
General Stability | Organism |
---|---|
treatment with ammonium sulfate during purification leads to a significant loss of activity | Achromobacter superficialis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Methyl-2-benzothiazolone hydrazone hydrochloride | weak inhibition | Achromobacter superficialis | |
D-cycloserine | 0.5 mM; 20% inhibition | Achromobacter superficialis | |
HgCl2 | - |
Achromobacter superficialis | |
hydroxylamine | - |
Achromobacter superficialis | |
L-Cycloserine | - |
Achromobacter superficialis | |
additional information | not inhibited by p-chloromercuribenzoate, monoiodoacetic acid, D-penicillamine, L-penicillamine and EDTA | Achromobacter superficialis | |
phenylhydrazine | 0.5 mM, 17% inhibition | Achromobacter superficialis | |
Semicarbazide | 0.5 mM, 21% inhibition | Achromobacter superficialis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
2-oxoglutarate | pH 8.0, 37°C, cosubstrate DL-3-aminoisobutyrate | Achromobacter superficialis | |
12 | - |
taurine | pH 8.0, 37°C | Achromobacter superficialis | |
16 | - |
Hypotaurine | pH 8.0, 37°C | Achromobacter superficialis | |
17 | - |
beta-Alanine | pH 8.0, 37°C | Achromobacter superficialis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
156000 | - |
sedimentation equilibrium | Achromobacter superficialis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter polymorph | - |
KR B-88 | - |
Achromobacter polymorph KR B-88 | - |
KR B-88 | - |
Achromobacter superficialis | - |
ICR-B-89 | - |
Achromobacter superficialis ICR-B-89 | - |
ICR-B-89 | - |
Purification (Comment) | Organism |
---|---|
- |
Achromobacter superficialis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.519 | - |
- |
Achromobacter superficialis |
Storage Stability | Organism |
---|---|
0-5°C, crystalline enzyme in 10 mM potassium phosphate buffer, pH 7.0, 60% saturation of ammonium sulfate, 100 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, several months, no loss of activity | Achromobacter superficialis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropanesulfonate + 2-oxoglutarate | - |
Achromobacter polymorph | 2-oxopropanesulfonate + L-glutamate | - |
? | |
3-aminopropanesulfonate + 2-oxoglutarate | 43% of the activity with taurine | Achromobacter superficialis | 2-oxopropanesulfonate + L-glutamate | - |
? | |
4-aminobutyrate + 2-oxoglutarate | 60% of the activity with taurine | Achromobacter superficialis | L-glutamate + acetaldehyde + sulfite | - |
? | |
beta-alanine + 2-oxoglutarate | - |
Achromobacter polymorph | 3-oxopropanoate + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | 184% of the activity with taurine | Achromobacter superficialis | 3-oxopropanoate + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | - |
Achromobacter polymorph KR B-88 | 3-oxopropanoate + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | 184% of the activity with taurine | Achromobacter superficialis ICR-B-89 | 3-oxopropanoate + L-glutamate | - |
? | |
DL-3-aminobutyrate + 2-oxoglutarate | 14% of the activity with taurine | Achromobacter superficialis | 3-oxobutyrate + L-glutamate | - |
? | |
DL-3-aminoisobutyrate + 2-oxoglutarate | - |
Achromobacter polymorph | 2-methyl-3-oxopropanoate + L-glutamate | - |
? | |
DL-3-aminoisobutyrate + 2-oxoglutarate | 208% of the activity with taurine | Achromobacter superficialis | 2-methyl-3-oxopropanoate + L-glutamate | - |
? | |
DL-3-aminoisobutyrate + 2-oxoglutarate | - |
Achromobacter polymorph KR B-88 | 2-methyl-3-oxopropanoate + L-glutamate | - |
? | |
DL-3-aminoisobutyrate + 2-oxoglutarate | 208% of the activity with taurine | Achromobacter superficialis ICR-B-89 | 2-methyl-3-oxopropanoate + L-glutamate | - |
? | |
hypotaurine + 2-oxoglutarate | - |
Achromobacter polymorph | 2-oxoethanesulfinic acid + L-glutamate | - |
? | |
hypotaurine + 2-oxoglutarate | 601% of the activity with taurine | Achromobacter superficialis | 2-oxoethanesulfinic acid + L-glutamate | - |
? | |
hypotaurine + 2-oxoglutarate | - |
Achromobacter polymorph KR B-88 | 2-oxoethanesulfinic acid + L-glutamate | - |
? | |
taurine + 2-oxoglutarate | no activity with aminomethanesulfonate, glycine, 5-aminopentanoate and amines | Achromobacter superficialis | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with L- and D-amino acids | Achromobacter polymorph | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with L- and D-amino acids | Achromobacter superficialis | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with pyruvate, phenylpyruvate, oxaloacetate | Achromobacter polymorph | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with pyruvate, phenylpyruvate, oxaloacetate | Achromobacter superficialis | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with L- and D-amino acids | Achromobacter polymorph KR B-88 | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with pyruvate, phenylpyruvate, oxaloacetate | Achromobacter polymorph KR B-88 | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with aminomethanesulfonate, glycine, 5-aminopentanoate and amines | Achromobacter superficialis ICR-B-89 | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with L- and D-amino acids | Achromobacter superficialis ICR-B-89 | sulfoacetaldehyde + L-glutamate | - |
r | |
taurine + 2-oxoglutarate | no activity with pyruvate, phenylpyruvate, oxaloacetate | Achromobacter superficialis ICR-B-89 | sulfoacetaldehyde + L-glutamate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Achromobacter superficialis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | - |
Achromobacter superficialis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km: 0.005 mM | Achromobacter superficialis | |
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Achromobacter polymorph | |
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Achromobacter superficialis |