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Literature summary for 2.6.1.52 extracted from
- Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies (2006), Proteins, 63, 742-753.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion method at 22°C crystal. Structure of phosphoserine aminotransferase is determined at 1.2 and 1.5 A resolution at pH 8.5 and 4.6, respectively | Niallia circulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
3-phosphonooxypyruvate | pH 9.0 | Niallia circulans |  |
| 0.1 | - |
3-phosphonooxypyruvate | pH 7.0 | Niallia circulans | |
| 0.2 | - |
L-glutamate | pH 9.0 | Niallia circulans | |
| 1 | - |
L-glutamate | pH 7.0 | Niallia circulans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q59196 | ssp. alkalophilus, recombinant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phospho-L-serine + 2-oxoglutarate | - |
Niallia circulans | 3-phosphonooxypyruvate + L-glutamate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BCIR PSAT | - |
Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
release of pyridoxal-5'-phosphate and protein thermal denaturation occur simultaneously at pH 6.0 in contrast to pH 8.5 where denaturation preceds release of cofactors by approximately 3°C | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
pH 8.5, Tm: 58.4°C | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
phosphoserine synthesis | Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | pH 8.0: about 50% of maximal activity, pH 9: about 60% of maximal activity, phosphoserine synthesis | Niallia circulans |
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