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Literature summary for 2.6.1.52 extracted from
- Molecular characterization of plastidic phosphoserine aminotransferase in serine biosynthesis from Arabidopsis (1998), Plant J., 16, 443-452.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
| cDNA encoding PSAT, single gene mapped on the lower arm of chromosome 4, overexpressed in Escherichia coli AD494 and BL21 | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-cysteine | - |
Arabidopsis thaliana |  |
| L-cysteine | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
L-glutamate | pH 8.2, 25°C | Arabidopsis thaliana | |
| 0.15 | - |
L-glutamate | pH 8.2, 25°C | Spinacia oleracea | |
| 3 | - |
3-phosphohydroxypyruvate | pH 8.2, 25°C | Spinacia oleracea | |
| 5 | - |
3-phosphohydroxypyruvate | pH 8.2, 25°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Spinacia oleracea | 9536 | - |
| plastid | - |
Arabidopsis thaliana | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + 3-phosphohydroxypyruvate | Spinacia oleracea | - |
O-phospho-L-serine + 2-oxoglutarate | - |
r | |
| L-glutamate + 3-phosphohydroxypyruvate | Arabidopsis thaliana | - |
O-phospho-L-serine + 2-oxoglutarate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q96255 | ecotype Columbia | - |
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| shoot | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + 3-phosphohydroxypyruvate | - |
Spinacia oleracea | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
| L-glutamate + 3-phosphohydroxypyruvate | - |
Arabidopsis thaliana | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
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