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Literature summary for 2.6.1.51 extracted from

  • Noguchi, T.; Okuno, E.; Kido, R.
    Identity of isoenzyme 1 of histidine-pyruvate aminotransferase with serine-pyruvate aminotransferase (1976), Biochem. J., 159, 607-613.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.51

Inhibitors

Inhibitors Comment Organism Structure
histidine competitive inhibitor of serine transamination Rattus norvegicus
Isonicotinic acid hydrazide
-
 Rattus norvegicus
KCN
-
 Rattus norvegicus
Semicarbazide
-
 Rattus norvegicus
serine competitive inhibitor of histidine transamination Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Rattus norvegicus
73000
-
 gel filtration Rattus norvegicus
75000
-
 sucrose density gradient centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + pyruvate Rattus norvegicus
-
 3-hydroxypyruvate + L-alanine
-
 r
L-serine + pyruvate Rattus norvegicus Donryu
-
 3-hydroxypyruvate + L-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Donryu strain
-
Rattus norvegicus Donryu
-
 Donryu strain
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme 1 of histidine-pyruvate aminotransferase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.47
-
 serine-pyruvate aminotransferase activity Rattus norvegicus
25.7
-
 histidine-pyruvate aminotransferase activity Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.5 may be stored for at least 6 weeks without loss of either activity Rattus norvegicus
0-5°C, little or none of either activity is lost when the enzyme is stored for 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxytryptophan + pyruvate
-
 Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + alanine
-
 ?
asparagine + pyruvate
-
 Rattus norvegicus 2-oxosuccinamate + L-alanine
-
 ?
glutamine + phenylpyruvate
-
 Rattus norvegicus 4-carbamoyl-2-oxobutanoate + L-phenylalanine
-
 ?
glutamine + pyruvate
-
 Rattus norvegicus 4-carbamoyl-2-oxobutanoate + L-alanine
-
 ?
glycine + 3-hydroxypyruvate
-
 Rattus norvegicus L-serine + glyoxylate
-
 r
histidine + pyruvate
-
 Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
L-alanine + phenylpyruvate
-
 Rattus norvegicus pyruvate + L-phenylalanine
-
 ?
L-asparagine + phenylpyruvate
-
 Rattus norvegicus 2-oxosuccinamate + L-phenylalanine
-
 ?
L-leucine + phenylpyruvate
-
 Rattus norvegicus 4-methyl-2-oxopentanoate + L-phenylalanine
-
 ?
L-methionine + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 ?
L-methionine + phenylpyruvate
-
 Rattus norvegicus Donryu 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 ?
L-methionine + pyruvate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
 ?
L-phenylalanine + pyruvate
-
 Rattus norvegicus phenylpyruvate + L-alanine
-
 ?
L-phenylalanine + pyruvate
-
 Rattus norvegicus Donryu phenylpyruvate + L-alanine
-
 ?
L-serine + oxaloacetate
-
 Rattus norvegicus 3-hydroxypyruvate + L-aspartate
-
 ?
L-serine + pyruvate
-
 Rattus norvegicus 3-hydroxypyruvate + L-alanine
-
 r
L-serine + pyruvate
-
 Rattus norvegicus Donryu 3-hydroxypyruvate + L-alanine
-
 r
L-tryptophan + pyruvate
-
 Rattus norvegicus 3-indole-2-oxopropanoate + L-alanine
-
 ?
L-tyrosine + pyruvate
-
 Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
-
 ?
L-tyrosine + pyruvate
-
 Rattus norvegicus Donryu 3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
-
 ?
additional information identical with histidine-pyruvate aminotransferase isoenzyme 1, isoleucine and oxoglutarate are no substrates Rattus norvegicus ?
-
 ?
additional information identical with histidine-pyruvate aminotransferase isoenzyme 1, isoleucine and oxoglutarate are no substrates Rattus norvegicus Donryu ?
-
 ?
ornithine + phenylpyruvate
-
 Rattus norvegicus 5-amino-2-oxopentanoate + L-phenylalanine
-
 ?
ornithine + pyruvate
-
 Rattus norvegicus 5-amino-2-oxopentanoate + L-alanine
-
 ?
threonine + phenylpyruvate
-
 Rattus norvegicus 3-hydroxy-2-oxobutanoate + L-phenylalanine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
isoenzyme 1 of histidine-pyruvate aminotransferase
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 heating of the purified enzyme for different lengths of time produces equivalent losses of both activities Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Rattus norvegicus