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Literature summary for 2.6.1.45 extracted from
- Serine-glyoxylate aminotransferase from kidney bean (Phaseolus vulgaris). II. The reverse reactions (1974), Biochim. Biophys. Acta, 370, 96-101.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 0.01 mM, 85% inhibition of glycin-hydroxypyruvate transamination | Phaseolus vulgaris |  |
| N-ethylmaleimide | - |
Phaseolus vulgaris | |
| NH4Cl | glycine-hydroxypyruvate transamination is only weakly inhibited | Phaseolus vulgaris | |
| p-hydroxymercuribenzoate | - |
Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.63 | - |
3-hydroxypyruvate | pH 8.0, 30°C, cosubstrate L-alanine | Phaseolus vulgaris | |
| 1.1 | - |
3-hydroxypyruvate | pH 8.0, 30°C, cosubstrate glycine | Phaseolus vulgaris | |
| 11 | - |
glycine | pH 8.0, 30°C | Phaseolus vulgaris | |
| 20 | - |
L-alanine | pH 8.0, 30°C | Phaseolus vulgaris | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + glyoxylate | Phaseolus vulgaris | involved in glycine metabolism | 3-hydroxypyruvate + glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
kidney bean, cv. Red Kidney | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + glyoxylate | reverse reaction at 4-11% the rate of forward reaction | Phaseolus vulgaris | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | preferred substrates | Phaseolus vulgaris | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | involved in glycine metabolism | Phaseolus vulgaris | 3-hydroxypyruvate + glycine | - |
? | |
| L-serine + pyruvate | 6% of glyoxylate transamination activity | Phaseolus vulgaris | 3-hydroxypyruvate + L-alanine | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
substrates L-alanine and 3-hydroxypyruvate | Phaseolus vulgaris |
| 9 | - |
substrates glycine and 3-hydroxypyruvate | Phaseolus vulgaris |
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