Crystallization (Comment) | Organism |
---|---|
mutant S187F, to a resolution of 2.9 A. The overall conformation of the variant is similar to that of normal AGTwith a displacement of the PLP-binding Lys209 and Val185, located on the re and si side of PLP, respectively, and slight conformational changes of other active site residues, in particular Trp108, the base stacking residue with the pyridine cofactor moiety. This results in a mispositioning of the AGT-pyridoxamine 5'-phosphate complex and of the external aldimine | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S187F | mutation gives rise to a variant associated with primary hyperoxaluria type I. Mutation shows a 300- to 500fold decrease in both the rate constant of L-alanine half-transamination and the kcat of the overall transamination, a different pyridoxamine 5'-phosphate binding mode and affinity, and a different microenvironment of the external aldimine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
glyoxylate | pH 7.4, 25°C | Homo sapiens | |
52 | - |
L-alanine | pH 7.4, 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P21549 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | - |
Homo sapiens | pyruvate + glycine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
AGXT | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.147 | - |
L-alanine | pH 7.4, 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |