Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F152I | soluble, catalytically active | Homo sapiens |
G41R | naturally occuring mutation, predicted to be responsible for the depletion of immunoreactive enzyme protein and formation of intraperoxisomal aggregates | Homo sapiens |
G41V | naturally occuring mutation | Homo sapiens |
G82E | naturally occuring mutation, decreased catalytic activity | Homo sapiens |
P11L/I340M | minor allele, naturally occuring variant; mutant protein (minor allel) is about 95% peroxisomal and 5% mitochondrial; P11/I340 major allele is 100% peroxisomal, minor allele in both the holo and apo forms is more sensitive to thermal denaturation and to urea unfolding than major allele | Homo sapiens |
P11L/I340M/F152I | naturally occuring mutation, possibly mistargeting into mitochondrial matrix | Homo sapiens |
P11L/I340M/G41R | naturally occuring mutation, predicted to be responsible for the depletion of immunoreactive enzyme protein and formation of intraperoxisomal aggregates | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
glyoxylate | G41V, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.15 | - |
glyoxylate | G82E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.22 | - |
glyoxylate | P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.23 | - |
glyoxylate | major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.25 | - |
glyoxylate | P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.28 | - |
glyoxylate | F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.32 | - |
glyoxylate | P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.41 | - |
glyoxylate | G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
15 | - |
L-alanine | G82E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
22 | - |
L-alanine | G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
28 | - |
L-alanine | P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
30 | - |
L-alanine | P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
31 | - |
L-alanine | major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
37 | - |
L-alanine | F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
41 | - |
L-alanine | P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
42 | - |
L-alanine | G41V, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | - |
Homo sapiens | pyruvate + glycine | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alanine:glyoxylate aminotransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
glyoxylate | G82E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.07 | - |
L-alanine | G82E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
10.6 | - |
L-alanine | P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
11.1 | - |
glyoxylate | P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
17.1 | - |
L-alanine | G41V, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
18.3 | - |
glyoxylate | G41V, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
19.8 | - |
L-alanine | G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
20.5 | - |
glyoxylate | G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
33 | - |
L-alanine | P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
33.6 | - |
L-alanine | P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
35 | - |
L-alanine | F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
37 | - |
glyoxylate | P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
39 | - |
glyoxylate | F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
40 | - |
glyoxylate | P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
45 | - |
glyoxylate | major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
45 | - |
L-alanine | major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 2 mol per dimer | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | deficiency is responsible for Primary Hyperoxaluria Type 1, an autosomal recessive disorder | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
L-alanine | G82E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.35 | - |
L-alanine | P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.41 | - |
L-alanine | G41V, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.45 | - |
glyoxylate | G82E, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.82 | - |
L-alanine | P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.9 | - |
L-alanine | G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.95 | - |
L-alanine | F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
1.2 | - |
L-alanine | P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
1.4 | - |
L-alanine | major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
35 | - |
glyoxylate | P11L/I340M/G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
50 | - |
glyoxylate | G41R, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
139 | - |
glyoxylate | F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
143 | - |
glyoxylate | G41V, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
160 | - |
glyoxylate | P11L/I340M/F152I, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
168 | - |
glyoxylate | P11L/I340M minor allele, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
196 | - |
glyoxylate | major allele (P11, I340), pH not specified in the publication, temperature not specified in the publication | Homo sapiens |