Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amino-oxyacetic acid | - |
Saccharomyces cerevisiae | |
hydroxylamine | - |
Saccharomyces cerevisiae | |
Isonicotinic acid hydrazide | - |
Saccharomyces cerevisiae | |
Semicarbazide | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
glyoxylate | pH 8.2, 37°C, isoenzyme 2, alanine as amino donor | Saccharomyces cerevisiae | |
2.1 | - |
L-alanine | pH 8.2, 37°C, glyoxylate as amino acceptor | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Saccharomyces cerevisiae |
80000 | - |
sucrose-density-gradient centrifugation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | Saccharomyces cerevisiae | regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates | pyruvate + glycine | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
101D, commercial baker's yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180.2 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 25 mM-potassium phosphate buffer, pH 7.5, containing 100 mM NaCl, 0.1 mM pyridoxal 5'-phosphate and 10% glycerol, my be stored for at least 3 months without loss of activity | Saccharomyces cerevisiae |
0-4°C, little or no activity is lost when stored for 2 weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + glyoxylate | - |
Saccharomyces cerevisiae | pyruvate + glycine | - |
ir | |
L-alanine + glyoxylate | regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates | Saccharomyces cerevisiae | pyruvate + glycine | - |
ir | |
additional information | utilizes only glyoxylate as amino acceptor, hydroxypyruvate, 2-oxoglutarate, phenylpyruvate and 2-oxo-4-methyl-thiobutyrate are inactive, only L-alanine as amino donor, L-serine, L-threonine, L-glutamic acid, L-glutamine, L-aspartic acid, L-asparagine, L-ornithine, L-leucine, L-valine, L-isoleucine, L-histidine, L-phenylalanine, L-tryptophan and L-tyrosine are no substrates | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | isoelectric focusing of the crude extract on a pH 3.0-10 pharmalyte gradient, 3 activity peaks with pI 7.4, 7.8 and 8.3 | - |
7 |