Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 5 mM 2-oxoglutarate inhibits the transamination of the 6.3-fold purified enzyme, of valine and isoleucine by about 50%, but the leucine activity is less affected. Inhibition is competitive with respect to valine | Methanococcus aeolicus | |
L-cysteine | 10 mM, inhibits activity with isoleucine and tyrosine by 22 and 34%, respectively | Methanococcus aeolicus | |
L-isoleucine | with 1 mM tyrosine as the substrate, 10 mM isoleucine inhibits activity by 94% | Methanococcus aeolicus | |
L-leucine | with 1 mM tyrosine as the substrate, 10 mM leucine inhibits activity by 93% | Methanococcus aeolicus | |
L-methionine | 10 mM methionine inhibits the activity with 1 mM tyrosine and phenylalanine by 86 and 59%, respectively, but it has no effect on the isoleucine activity | Methanococcus aeolicus | |
L-phenylalanine | with 2 mM isoleucine as the substrate, 10 mM phenylalanine inhibits activity by 46% | Methanococcus aeolicus | |
L-valine | with 1 mM tyrosine as the substrate, 10 mM valine inhibits activity by 78% | Methanococcus aeolicus | |
additional information | not inhibited by 5 mM KCI, NaCl, CuCl2, NiCl2, CoCl2, or FeCl2. No of activity with tyrosine by 10 mM aspartate, arginine, asparagine, glutamine, glycine, histidine, lysine, proline, serine, threonine or alanine | Methanococcus aeolicus | |
ZnCl2 | 5 mM, 50% inhibition | Methanococcus aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-phenylalanine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
0.1 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-phenylalanine | Methanococcus aeolicus | |
0.1 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-tryptophan | Methanococcus aeolicus | |
0.1 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-tyrosine | Methanococcus aeolicus | |
0.3 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-isoleucine | Methanococcus aeolicus | |
0.3 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-valine | Methanococcus aeolicus | |
0.6 | - |
2-oxoglutarate | pH and temperature not specified in the publication, cosubstrate: L-leucine | Methanococcus aeolicus | |
1.1 | - |
L-tyrosine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
1.1 | - |
L-leucine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
1.4 | - |
L-isoleucine | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.8 | - |
L-tryptophan | pH and temperature not specified in the publication | Methanococcus aeolicus | |
2.8 | - |
L-valine | pH and temperature not specified in the publication | Methanococcus aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM MgCl2 stimulates by 26% | Methanococcus aeolicus | |
Mn2+ | 5 mM MgCl2 stimulates by 34% | Methanococcus aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
188000 | - |
gel filtration | Methanococcus aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Methanococcus aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Methanococcus aeolicus | L-glutamate + 3-methyl-2-oxopentanoate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Methanococcus aeolicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Methanococcus aeolicus | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Methanococcus aeolicus | L-glutamic acid + 3-indole-2-oxopropanoate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Methanococcus aeolicus | p-hydroxyphenylpyruvate + L-glutamate | - |
? | |
L-valine + 2-oxoglutarate | - |
Methanococcus aeolicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Methanococcus aeolicus |
branched-chain aminotransferase | - |
Methanococcus aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanococcus aeolicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
2-oxoglutarate | 6.3-fold purified enzyme, pH and temperature not specified in the publication | Methanococcus aeolicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanococcus aeolicus | isoelectric focusing | - |
4.9 |