Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.4 extracted from

  • Donini, S.; Ferrari, M.; Fedeli, C.; Faini, M.; Lamberto, I.; Marletta, A.S.; Mellini, L.; Panini, M.; Percudani, R.; Pollegioni, L.; Caldinelli, L.; Petrucco, S.; Peracchi, A.
    Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism (2009), Biochem. J., 422, 265-272.
    View publication on PubMed
Search for more literature for 2.6.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as His6-tagged protein in Escherichia coli cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 glyoxylate pH 7.4, 37°C, cosubstrate: L-glutamate Homo sapiens
0.81
-
 glyoxylate pH 7.4, 37°C, cosubstrate: L-alanine Homo sapiens
5.5
-
 glyoxylate pH 7.4, 37°C, cosubstrate: L-glutamate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 recombinant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P24298
-
-
Homo sapiens Q9Y617
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
 pH 7.4, 25°C, L-glutamate (1 mM) + glyoxylate Homo sapiens
2.1
-
 pH 7.4, 25°C, L-alanine (10 mM) + glyoxylate Homo sapiens
2.5
-
 pH 7.4, 25°C, L-glutamate (10 mM) + glyoxylate Homo sapiens
87
-
 pH 7.5, 25°C, L-alanine + 2-oxoglutarate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
 Homo sapiens pyruvate + L-glutamate
-
 ?
L-alanine + glyoxylate
-
 Homo sapiens pyruvate + glycine
-
 ?
L-alanine + glyoxylate L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate Homo sapiens pyruvate + glycine
-
 ?
L-asparagine + glyoxylate
-
 Homo sapiens 4-amino-2,4-dioxobutanoate + glycine
-
 ?
L-glutamate + glyoxylate L-Glu is the preferred amino-group donor both at 25°C and at 37°C Homo sapiens 2-oxoglutarate + glycine
-
 ?
L-glutamate + glyoxylate L-glutamate and L-alanine are by far the best amino-group donors for glyoxylate Homo sapiens 2-oxoglutarate + glycine
-
 ?
L-homocysteate + glyoxylate
-
 Homo sapiens ? + glycine
-
 ?
L-serine + glyoxylate
-
 Homo sapiens 3-hydroxy-2-oxopropanoate + glycine
-
 ?
additional information phosphoserine is not an efficient amino-group donor Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GPT
-
 Homo sapiens
PSAT1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens