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Literature summary for 2.6.1.39 extracted from

  • Okuno, E.; Tsujimoto, M.; Nakamura, M.; Kido, R.
    2-Aminoadipate-2-oxoglutarate aminotransferase isoenzymes in human liver: a plausible physiological role in lysine and tryptophan metabolism (1993), Enzyme Protein, 47, 136-148.
    View publication on PubMed
Search for more literature for 2.6.1.39

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 L-2-aminoadipate pH 8.0, 37°C isoenzyme AadAT-II Homo sapiens
1
-
 2-oxoadipate pH 8.0, 37°C isoenzyme AadAT-II Homo sapiens
1.1
-
 2-oxoglutarate pH 8.0, 37°C isoenzyme AadAT-I Homo sapiens
1.4
-
 glutamate pH 8.0, 37°C Homo sapiens
2.5
-
 2-oxoadipate pH 8.0, 37°C isoenzyme AadAT-I Homo sapiens
3.2
-
 2-oxoglutarate pH 8.0, 37°C isoenzyme AadAT-II Homo sapiens
12.5
-
 L-glutamate pH 8.0, 37°C, isoenzyme AadAT-II Homo sapiens
20
-
 L-2-aminoadipate pH 8.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm isoenzyme AadAT-I Homo sapiens 5737
-
cytosol
-
 Homo sapiens 5829
-
mitochondrion isoenzyme AadAT-II Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 2 * 46000, SDS-PAGE Homo sapiens
98000
-
 isoenzyme AadAT-II, sucrose density gradient centrifugation Homo sapiens
104000
-
 isoenzyme AadAT-I, sucrose density gradient centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-aminoadipate + 2-oxoglutarate Homo sapiens lysine and tryptophan metabolism 2-oxoadipate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
2 isoenzymes, AadAT-I and AadAT-II Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.77
-
 isoenzyme AadAT-I Homo sapiens
10.68
-
 isoenzyme AadAT-II Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kynurenine + 2-oxoglutarate
-
 Homo sapiens kynurenic acid + L-glutamate
-
 ir
L-2-aminoadipate + 2-oxoglutarate
-
 Homo sapiens 2-oxoadipate + L-glutamate
-
 r
L-2-aminoadipate + 2-oxoglutarate lysine and tryptophan metabolism Homo sapiens 2-oxoadipate + L-glutamate
-
 r
L-tryptophan + 2-oxoglutarate
-
 Homo sapiens 3-indole-2-oxopropanoate + L-glutamate
-
 ?
additional information 2 isoenzymes, AadAT-I and AadAT-II, isoenzyme AadAT-II shows additional activity with tryptophan or kynurenine-2-oxoglutarate reaction, only slight activity with asparagine, alanine, arginine, ornithine, isoleucine, valine, leucine, lysine, serine, threonine, phenylalanine, tyrosine, histidine, glutamine, methionine or aspartate with 2-oxoglutarate Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
AadAT
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5 isoenzyme AadAT-II Homo sapiens
9.5
-
 isoenzyme AadAT-I Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
9 9.5 isoenzyme AadAT-I, sensitive to pH changes, activity is quickly lost at a higher pH, isoenzyme AadAT-II is less sensitive to pH changes Homo sapiens