Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.37 extracted from

  • Kim, A.D.; Baker, A.S.; Dunaway-Mariano, D.; Metcalf, W.W.; Wanner, B.L.; Martin, B.M.
    The 2-aminoethylphosphonate-specific transaminase of the 2-aminoethylphosphonate degradation pathway (2002), J. Bacteriol., 184, 4134-4140.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Salmonella enterica

Protein Variants

Protein Variants Comment Organism
D168A no activity Salmonella enterica
K194L no activity Salmonella enterica
K194R no activity Salmonella enterica
R340A partial activity Salmonella enterica
R340K partial activity Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
0.15
-
pyruvate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde Salmonella enterica
0.19
-
2-phosphonoacetaldehyde pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
0.5
-
pyruvate pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
1.11
-
(2-aminoethyl)phosphonate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde Salmonella enterica
1.4
-
L-alanine pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
2.8
-
D-alanine pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
2.9
-
2-phosphonoacetaldehyde pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
3.7
-
D-alanine pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
6.1
-
pyruvate pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
11
-
D-alanine pH 8.5, 25°C Salmonella enterica
20
-
L-alanine pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
26
-
2-aminoethylphosphonate pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
140
-
L-alanine pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16500
-
4 * 16500, SDS-PAGE Pseudomonas aeruginosa
42000
-
2 * 42000, SDS-PAGE Salmonella enterica
100000
-
gel filtration Salmonella enterica

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q51573 encoded by phnW gene
-
Salmonella enterica
-
serovar Thyphimurium
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, amonium sulfate, DEAE-cellulose Salmonella enterica

Reaction

Reaction Comment Organism Reaction ID
(2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine bi-bi ping-pong mechanism Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-aminoethyl)phosphonate + pyruvate highly specific for (2-aminoethyl)phosphonate, no activity with oxaloacetate as NH3 donor Salmonella enterica 2-phosphonoacetaldehyde + L-alanine
-
r
2-phosphonoacetaldehyde + D-alanine L-alanine is preferred but not absolutely required Salmonella enterica (2-aminoethyl)phosphonate + pyruvate
-
?
alpha-ketoglutarate + L-alanine 0.25% of the kcat observed with 2-phosphonoacetaldehyde Salmonella enterica L-glutamate + pyruvate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Salmonella enterica
tetramer 4 * 16500, SDS-PAGE Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
(2-aminoethyl)phosphonate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde, R340K and R340A mutant enzymes Salmonella enterica
0.02
-
2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340A mutant enzyme Salmonella enterica
0.04
-
D-alanine pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
0.6
-
2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340K mutant enzyme Salmonella enterica
7.4
-
(2-aminoethyl)phosphonate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde Salmonella enterica
9.3
-
L-alanine pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
9.3
-
2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9.5 synthesis of 2-phosphonoacetaldehyde Salmonella enterica
7.5 8.5 synthesis of (2-aminoethyl)phosphonate Salmonella enterica