Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
D168A | no activity | Salmonella enterica |
K194L | no activity | Salmonella enterica |
K194R | no activity | Salmonella enterica |
R340A | partial activity | Salmonella enterica |
R340K | partial activity | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
2-phosphonoacetaldehyde | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate | Salmonella enterica | |
0.15 | - |
pyruvate | pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde | Salmonella enterica | |
0.19 | - |
2-phosphonoacetaldehyde | pH 8.5, 25°C, R340A mutant enzyme | Salmonella enterica | |
0.5 | - |
pyruvate | pH 8.5, 25°C, R340K mutant enzyme | Salmonella enterica | |
1.11 | - |
(2-aminoethyl)phosphonate | pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde | Salmonella enterica | |
1.4 | - |
L-alanine | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate | Salmonella enterica | |
2.8 | - |
D-alanine | pH 8.5, 25°C, R340K mutant enzyme | Salmonella enterica | |
2.9 | - |
2-phosphonoacetaldehyde | pH 8.5, 25°C, R340K mutant enzyme | Salmonella enterica | |
3.7 | - |
D-alanine | pH 8.5, 25°C, R340A mutant enzyme | Salmonella enterica | |
6.1 | - |
pyruvate | pH 8.5, 25°C, R340A mutant enzyme | Salmonella enterica | |
11 | - |
D-alanine | pH 8.5, 25°C | Salmonella enterica | |
20 | - |
L-alanine | pH 8.5, 25°C, R340K mutant enzyme | Salmonella enterica | |
26 | - |
2-aminoethylphosphonate | pH 8.5, 25°C, R340A mutant enzyme | Salmonella enterica | |
140 | - |
L-alanine | pH 8.5, 25°C, R340A mutant enzyme | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16500 | - |
4 * 16500, SDS-PAGE | Pseudomonas aeruginosa |
42000 | - |
2 * 42000, SDS-PAGE | Salmonella enterica |
100000 | - |
gel filtration | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q51573 | encoded by phnW gene | - |
Salmonella enterica | - |
serovar Thyphimurium | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, amonium sulfate, DEAE-cellulose | Salmonella enterica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine | bi-bi ping-pong mechanism | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-aminoethyl)phosphonate + pyruvate | highly specific for (2-aminoethyl)phosphonate, no activity with oxaloacetate as NH3 donor | Salmonella enterica | 2-phosphonoacetaldehyde + L-alanine | - |
r | |
2-phosphonoacetaldehyde + D-alanine | L-alanine is preferred but not absolutely required | Salmonella enterica | (2-aminoethyl)phosphonate + pyruvate | - |
? | |
alpha-ketoglutarate + L-alanine | 0.25% of the kcat observed with 2-phosphonoacetaldehyde | Salmonella enterica | L-glutamate + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Salmonella enterica |
tetramer | 4 * 16500, SDS-PAGE | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(2-aminoethyl)phosphonate | pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde, R340K and R340A mutant enzymes | Salmonella enterica | |
0.02 | - |
2-phosphonoacetaldehyde | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340A mutant enzyme | Salmonella enterica | |
0.04 | - |
D-alanine | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate | Salmonella enterica | |
0.6 | - |
2-phosphonoacetaldehyde | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340K mutant enzyme | Salmonella enterica | |
7.4 | - |
(2-aminoethyl)phosphonate | pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde | Salmonella enterica | |
9.3 | - |
L-alanine | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate | Salmonella enterica | |
9.3 | - |
2-phosphonoacetaldehyde | pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | synthesis of 2-phosphonoacetaldehyde | Salmonella enterica |
7.5 | 8.5 | synthesis of (2-aminoethyl)phosphonate | Salmonella enterica |