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Literature summary for 2.6.1.37 extracted from
- Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa (1983), Eur. J. Biochem., 133, 119-125.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminobutyrate | inhibitor/substrate ratio = 3, 8% inhibition | Pseudomonas aeruginosa |  |
| acetate | inhibitor/substrate ratio = 3, 20% inhibition | Pseudomonas aeruginosa | |
| Aminomethylphosphonate | inhibitor/substrate ratio = 3, 50% inhibition | Pseudomonas aeruginosa | |
| Aminomethylsulfonate | inhibitor/substrate ratio = 3, 20% inhibition | Pseudomonas aeruginosa | |
| Aminooxyacetate | 1 mM, 100% inhibition | Pseudomonas aeruginosa | |
| beta-Alanine | inhibitor/substrate ratio = 3, 20% inhibition | Pseudomonas aeruginosa | |
| D-cycloserine | 1 mM, 40% inhibition | Pseudomonas aeruginosa | |
| DL-1-Aminobutylphosphonate | inhibitor/substrate ratio = 3, 22% inhibition | Pseudomonas aeruginosa | |
| DL-1-Aminopentylphosphonate | inhibitor/substrate ratio = 3, 22% inhibition | Pseudomonas aeruginosa | |
| ethylphosphonate | inhibitor/substrate ratio = 3, 94% inhibition | Pseudomonas aeruginosa | |
| ethylsulfonate | inhibitor/substrate ratio = 3, 12% inhibition | Pseudomonas aeruginosa | |
| HgCl2 | 1 mM, 100% inhibition | Pseudomonas aeruginosa | |
| hydroxylamine | 1 mM, 50% inhibition | Pseudomonas aeruginosa | |
| L-cysteine | 10 mM, 35% inhibition | Pseudomonas aeruginosa | |
| methylphosphonate | inhibitor/substrate ratio = 3, 90% inhibition | Pseudomonas aeruginosa | |
| phenylhydrazine | 1 mM, 20% inhibition | Pseudomonas aeruginosa | |
| Propylphosphonate | inhibitor/substrate ratio = 3, 65% inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
pyruvate | pH 8.5, 30°C | Pseudomonas aeruginosa | |
| 3.85 | - |
2-aminoethylphosphonate | pH 8.5, 30°C | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16500 | - |
4 * 16500, SDS-PAGE | Pseudomonas aeruginosa |
| 65000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| poly(ethyleneimine), heat treatment, ammonium sulfate, DEAE-cellulose, hydroxyapatite, Ultrogel AcA | Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine | ping-pong bi-bi mechanism | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.64 | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months, no loss of activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-aminoethyl)phosphonate + pyruvate | highly specific for pyruvate and (2-aminoethyl)phosphonate, no activity with D-penicillamine, N-ethylmaleimide, iodoacetamide, 3-aminopropylphosphonate and taurine | Pseudomonas aeruginosa | 2-phosphonoacetaldehyde + L-alanine | - |
? | |
| (2-aminoethyl)phosphonate + pyruvate | trivial name ciliatine | Pseudomonas aeruginosa | 2-phosphonoacetaldehyde + L-alanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 16500, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
inactivation | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 10.8 | approx. 20% of maximal activity at pH 7.3, approx. 60% of maximal activity at pH 10.8, no activity above pH 11.5 | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | a pyridoxal phosphate protein, 4 mol of pyridoxal phosphate per mol of enzyme | Pseudomonas aeruginosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
ethylphosphonate | - |
Pseudomonas aeruginosa | |
| 1.55 | - |
methylphosphonate | - |
Pseudomonas aeruginosa | |
| 2.8 | - |
Propylphosphonate | - |
Pseudomonas aeruginosa | |
| 3.85 | - |
Aminomethylphosphonate | - |
Pseudomonas aeruginosa | |
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