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Literature summary for 2.6.1.36 extracted from

  • Soda, K.; Misono, H.
    L-Lysine:alpha-ketoglutarate aminotransferase. II. Purification, crystallization, and properties (1968), Biochemistry, 7, 4110-4119.
    View publication on PubMed
Search for more literature for 2.6.1.36

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 [Flavobacterium] lutescens

General Stability

General Stability Organism
pyridoxal 5'-phosphate, 0.1 mM, or 2-mercaptoethanol, 0.02%, protects against heat inactivation [Flavobacterium] lutescens

Inhibitors

Inhibitors Comment Organism Structure
additional information incubation of the enzyme with L-lysine in the presence of high concentrations of phosphate gives an inactive form of the enzyme (semiapoenzyme), reactivation with pyridoxal 5'-phosphate [Flavobacterium] lutescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 2-oxoglutarate cosubstrate L-ornithine, pH 8.0, 37°C [Flavobacterium] lutescens
0.5
-
 2-oxoglutarate L-lysine, pH 8.0, 37°C [Flavobacterium] lutescens
2
-
 L-ornithine pH 8.0, 37°C [Flavobacterium] lutescens
2.8
-
 L-lysine pH 8.0, 37°C [Flavobacterium] lutescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 sedimentation equilibrium method [Flavobacterium] lutescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate [Flavobacterium] lutescens
-
 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
[Flavobacterium] lutescens
-
 i.e. Achromobacter liquidum
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Flavobacterium] lutescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.67
-
 pH 8.0, 37°C [Flavobacterium] lutescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate
-
 [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?
L-lysine + 2-oxoglutarate 2-oxoglutarate is the only active amino acceptor [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?
L-ornithine + 2-oxoglutarate
-
 [Flavobacterium] lutescens ? + L-glutamate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 pyridoxal 5'-phosphate, 0.1 mM, or 2-mercaptoethanol, 0.02%, protects against heat inactivation [Flavobacterium] lutescens
55
-
 pH 6.0-7.5, 5 min, stable, partially purified enzyme [Flavobacterium] lutescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 substrates L-ornithine + 2-oxoglutarate [Flavobacterium] lutescens
8.3 8.5 substrates lysine + 2-oxoglutarate [Flavobacterium] lutescens

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 about 40% of maximum activity at pH 6 and pH 9.5, substrates ornithine + 2-oxoglutarate [Flavobacterium] lutescens
7 10 pH 7: about 50% of maximum activity, pH 10: about 30% of maximum activity [Flavobacterium] lutescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 55°C, 5 min, stable, partially purified enzyme [Flavobacterium] lutescens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal phosphate protein [Flavobacterium] lutescens
pyridoxal 5'-phosphate 2 mol of pyridoxal 5'-phosphate per mol of holoenzyme [Flavobacterium] lutescens
pyridoxal 5'-phosphate Km: 0.000357 mM [Flavobacterium] lutescens
pyridoxamine 5'-phosphate can replace pyridoxal 5'-phosphate, Km: 0.00715 mM [Flavobacterium] lutescens