Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | activation, less effective than phosphate | Rattus norvegicus | |
D-Aspartate | increases activity | Rattus norvegicus | |
diphosphate | activation, less effective than phosphate | Rattus norvegicus | |
phosphate | essential, activation in absence of diethylstilbestrol and pyridoxal 5'-phosphate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate | Rattus norvegicus | |
2-Oxomalonate | inhibition at pH 7.0 is higher than at pH 8.5, 0.4 mM in presence of equimolar concentrations of oxaloacetate | Rattus norvegicus | |
3,3-Dimethylglutamic acid | not at pH 7.0, weak at pH 8.0 | Rattus norvegicus | |
3-oxoadipate | inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate | Rattus norvegicus | |
adipic acid | not at pH 7.0, weak at pH 8.0 | Rattus norvegicus | |
Benzylmalonic acid | not at pH 7.0, weak at pH 8.0 | Rattus norvegicus | |
Diethylstilbestrol diphosphate | - |
Rattus norvegicus | |
L-aspartate | - |
Rattus norvegicus | |
L-glutamate | - |
Rattus norvegicus | |
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
pyridoxamine phosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
oxaloacetate | somewhat higher at very high levels of pyridoxamine, 14 mM | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + oxaloacetate | Rattus norvegicus | - |
pyridoxal + L-aspartate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
979fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.066 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
frozen, 20% loss of activity after 2 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + oxaloacetate | - |
Rattus norvegicus | pyridoxal + L-aspartate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | about 50% of activity maximum at pH 7.5 and pH 9.5 | Rattus norvegicus |