Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | preincubation, activates | Sus scrofa | |
additional information | no activation by: pyridoxine, pyridoxal, pyridoxamine | Sus scrofa | |
phosphate | maximal activity in presence of phosphate buffer, in absence reaction proceeds at 50% of that observed in optimal phosphate concentration | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Fluorophenylalanine | 32 mM, 50% inhibition | Sus scrofa | |
additional information | not affected by metal chelators and high concentration of ammonium sulfate; reduced activity in cacodylate, Tris and borate buffers | Sus scrofa | |
p-chloromercuribenzoate | reduced by preincubation with L-phenylalanine and pyridoxal phosphate and reversed by a subsequent preincubation with 2-mercaptoethanol | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
2-oxoglutarate | cosubstrate phenylalanine, pH 8.0, 37°C | Sus scrofa | |
3.8 | - |
L-tyrosine | pH 8.0, 37°C | Sus scrofa | |
6 | - |
L-3,4-dihydroxyphenylalanine | pH 8.0, 37°C | Sus scrofa | |
7 | - |
4-Fluorophenylalanine | pH 8.0, 37°C | Sus scrofa | |
15 | - |
L-tryptophan | pH 8.0, 37°C | Sus scrofa | |
50 | - |
L-phenylalanine | pH 8.0, 37°C | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
about 900fold | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | cortex | Sus scrofa | - |
brain cortex | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.04 | - |
reverse reaction, purified enzyme | Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 0.40 M potassium phosphate buffer, pH 8.0, highly purified enzyme stable for at least 2 weeks | Sus scrofa |
-20°C, 100fold purified enzyme, 0.4 M potassium phosphate, pH 8.0, stable for at least 9 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxytryptophan + 2-oxoglutarate | DL-5-hydroxytryptophan, 62% as effective as L-phenylalanine | Sus scrofa | 3-(5-hydroxyindole)-2-oxopropanoate + L-glutamate | - |
? | |
DL-p-fluorophenylalanine + 2-oxoglutarate | 41% as effective as phenylalanine | Sus scrofa | 3-(4-fluorophenyl)-2-oxopropanoate + L-glutamate | - |
? | |
L-3,4-dihydroxyphenylalanine + 2-oxoglutarate | 46% as effective as L-phenylalanine | Sus scrofa | 3-(3,4-dihydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
? | |
L-aspartate + phenylpyruvate | 10% as effective as L-glutamate | Sus scrofa | 2-oxosuccinic acid + L-phenylalanine | - |
r | |
L-histidine + 2-oxoglutarate | 35% as effective as L-phenylalanine | Sus scrofa | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Sus scrofa | L-glutamate + phenylpyruvate | - |
r | |
L-phenylalanine + 2-oxosuccinic acid | 70% as effective as 2-oxoglutarate | Sus scrofa | phenylpyruvate + L-aspartate | - |
r | |
L-tryptophan + 2-oxoglutarate | 52% of the activity with L-phenylalanine | Sus scrofa | L-glutamate + 3-indole-2-oxopropanoate | - |
? | |
L-tyrosine + 2-oxoglutarate | 49% of the activity with L-phenylalanine | Sus scrofa | L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate | - |
? | |
additional information | substrate specificity | Sus scrofa | ? | - |
? | |
additional information | no activity with D-phenylalanine and D-glutamic acid | Sus scrofa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sus scrofa |
8 | 9 | phenylalanine | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | pyridoxamine 5'-phosphate and pyridoxal phosphate are effective in partially restoring the apoenzyme activity and in stimulating the holoenzyme pyridoxamine phosphate, pyridoxamine phosphate is somewhat more effective than pyridoxal phosphate | Sus scrofa | |
pyridoxamine 5'-phosphate | pyridoxamine 5'-phosphate and pyridoxal phosphate are effective in partially restoring the apoenzyme activity and in stimulating the holoenzyme pyridoxamine phosphate, pyridoxamine phosphate is somewhat more effective than pyridoxal phosphate | Sus scrofa |