Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,5-Diiodo-4-hydroxyphenyl-DL-lactic acid | - |
Rattus norvegicus | |
3,5-Diiodo-4-hydroxyphenylacetate | strong inhibition, mixed type | Rattus norvegicus | |
4-chloromercuribenzoate | - |
Rattus norvegicus | |
4-hydroxyphenyl-DL-lactic acid | weak | Rattus norvegicus | |
4-hydroxyphenylacetic acid | - |
Rattus norvegicus | |
o-Iodosobenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | 1.3 | 3,5-diiodo-L-tyrosine | pH 6.2, 37°C | Rattus norvegicus | |
3.4 | 3.7 | 3,5-Dibromo-L-tyrosine | pH 6.5, 37°C | Rattus norvegicus | |
6.1 | - |
3,5-dichloro-L-tyrosine | pH 6.-L-7, 37°C | Rattus norvegicus | |
6.5 | - |
3-iodo-L-tyrosine | pH 7.4, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-diiodo-L-tyrosine + 2-oxoglutarate | Rattus norvegicus | - |
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
30fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
partially purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, 50 mM sodium phosphate buffer, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.15 M NaCl, approximately 4 mg protein/ml, 40% loss of activity after 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dibromo-L-tyrosine + 2-oxoglutarate | at 127% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | 3,5-dibromo-4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
3,5-dichloro-L-tyrosine + 2-oxoglutarate | at 110% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | 3,5-dichloro-4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
3,5-diiodo-L-tyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
3,5-diiodo-L-tyrosine + 2-oxoglutarate | specific for 2-oxoglutarate as amino acceptor | Rattus norvegicus | 3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
3,5-diiodo-L-tyrosine + oxaloacetate | 30% substitution of 2-oxoglutarate by oxaloacetate as amino acceptor | Rattus norvegicus | 3,5-diiodo-4-hydroxyphenylpyruvate + L-aspartate | - |
? | |
3-(4-fluorophenyl)alanine + 2-oxoglutarate | i.e. p-fluoro-DL-phenylalanine, at 3.1% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | (4-fluorophenyl)pyruvate + L-glutamate | - |
? | |
3-iodo-L-tyrosine + 2-oxoglutarate | at 76% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | 3-iodo-4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | at 3.9% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | at 3.6% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | at 3.9% the rate of 3,5-diiodo-L-tyrosine | Rattus norvegicus | (4-hydroxyphenyl)pyruvate + L-glutamate | - |
? | |
additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
additional information | no activity with: D-diiodotyrosine, tyrosine analogues with nitro, amino or hydroxy substituents in position 3 or 3 and 5 | Rattus norvegicus | ? | - |
? | |
additional information | no activity with pyruvate as amino acceptor | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
with substrate diiodo-L-tyrosine | Rattus norvegicus |
6.5 | - |
with substrate dibromo-L-tyrosine, dichloro-L-tyrosine | Rattus norvegicus |
7 | 7.5 | with substrate monoiodo-L-tyrosine | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Rattus norvegicus |