Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by pyridoxamine | Bacillus subtilis | |
pyridoxamine phosphate | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
sedimentation equilibrium centrifugation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, ammonium sulfate, DEAE-Sephadex, hydroxylapatite | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-10°C, 20 mM potassium phosphate, pH 7.0, 2 months, no loss of activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-aminobutanoate + pyruvate | - |
Bacillus subtilis | 2-oxobutanoate + D-alanine | - |
r | |
D-alanine + 2-oxobutanoate | - |
Bacillus subtilis | pyruvate + 2-aminobutanoate | - |
r | |
D-alanine + 2-oxoglutarate | D-alanine formation is favoured | Bacillus subtilis | pyruvate + D-glutamate | - |
r | |
D-aminobutanoate + 2-oxoglutarate | - |
Bacillus subtilis | oxobutanoate + D-glutamate | - |
? | |
D-asparagine + 2-oxoglutarate | - |
Bacillus subtilis | 2-oxosuccinamate + D-glutamate | - |
? | |
D-asparagine + pyruvate | - |
Bacillus subtilis | 2-oxosuccinamate + D-alanine | - |
? | |
D-aspartate + 2-oxoglutarate | - |
Bacillus subtilis | 2-oxosuccinate + D-glutamate | - |
? | |
D-aspartate + pyruvate | - |
Bacillus subtilis | 2-oxosuccinate + D-alanine | - |
? | |
D-glutamate + 2-oxobutanoate | - |
Bacillus subtilis | 2-oxoglutarate + 2-aminobutanoate | - |
? | |
D-glutamate + 2-oxoglutarate | - |
Bacillus subtilis | 2-oxoglutarate + D-glutamate | - |
? | |
D-glutamate + pyruvate | - |
Bacillus subtilis | 2-oxoglutarate + D-alanine | - |
r | |
D-methionine + 2-oxoglutarate | weak activity | Bacillus subtilis | 4-methylsulfanyl-2-oxobutanoate + D-glutamate | - |
? | |
D-methionine + pyruvate | - |
Bacillus subtilis | 4-methylsulfanyl-2-oxobutanoate + L-alanine | - |
? | |
D-norvaline + 2-oxoglutarate | - |
Bacillus subtilis | 2-oxopentanoate + D-glutamate | - |
? | |
D-norvaline + pyruvate | - |
Bacillus subtilis | 2-oxopentanoate + D-alanine | - |
? | |
D-ornithine + 2-oxoglutarate | weak activity | Bacillus subtilis | ? + D-glutamate | - |
? | |
D-ornithine + pyruvate | - |
Bacillus subtilis | ? + L-alanine | - |
? | |
D-serine + 2-oxoglutarate | weak activity | Bacillus subtilis | 3-hydroxy-2-oxopropanoate + D-glutamate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.8 | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | holoenzyme contains 1 mol of vitamin B6 per molecule of enzyme, 25-50% of maximal activity in the absence of pyridoxal 5-phosphate | Bacillus subtilis |