Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21(DE3) cells | Lachancea kluyveri |
expression as His-tagged protein | Saccharomyces cerevisiae |
expression as His-tagged protein | Schizosaccharomyces pombe |
expression as His-tagged protein | Lachancea kluyveri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
2-oxoglutarate | pH 8.0, 30°C | Lachancea kluyveri | |
0.22 | - |
2-oxoglutarate | in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate | Lachancea kluyveri | |
2.1 | - |
2-oxoglutarate | pH 8.0, 30°C | Lachancea kluyveri | |
3.2 | - |
gamma-aminobutanoate | pH 8.0, 30°C | Lachancea kluyveri | |
3.2 | - |
4-aminobutanoate | in 100 mM potassium phosphate (pH 8.0), 0.1 mM pyridoxal 5'-phosphate | Lachancea kluyveri | |
7.6 | - |
beta-Alanine | pH 8.0, 30°C | Lachancea kluyveri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Lachancea kluyveri |
98000 | - |
PAGE | Lachancea kluyveri |
111000 | - |
PAGE | Lachancea kluyveri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lachancea kluyveri | - |
- |
- |
Lachancea kluyveri | A5H0J5 | isoform Pyd4, involved in both beta-alanine and gamma-aminobutanoic acid transamination | - |
Lachancea kluyveri | A5H0J6 | isoform Uga1 | - |
Saccharomyces cerevisiae | P17649 | isoform Uga1 | - |
Schizosaccharomyces pombe | O13837 | isoform Uga1 | - |
Purification (Comment) | Organism |
---|---|
nickel-chelating Sepharose chromatography and Sepharose S12 gel filtration | Lachancea kluyveri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Lachancea kluyveri |
0.13 | - |
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
0.14 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Schizosaccharomyces pombe |
0.15 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Saccharomyces cerevisiae |
0.75 | - |
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
1.6 | - |
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
3.52 | - |
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30°C | Lachancea kluyveri |
3.6 | - |
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Saccharomyces cerevisiae |
3.67 | - |
continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Schizosaccharomyces pombe |
4.5 | - |
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
12.8 | - |
using 4-aminobutanoate as substrate | Lachancea kluyveri |
12.8 | - |
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30°C | Lachancea kluyveri |
Storage Stability | Organism |
---|---|
4°C, 12 days, 57% residual activity | Saccharomyces cerevisiae |
4°C, 12 days, 57% residual activity | Schizosaccharomyces pombe |
4°C, 12 days, 57% residual activity | Lachancea kluyveri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + 2-oxoglutarate | - |
Lachancea kluyveri | 4-oxobutanoate + L-glutamate | - |
r | |
4-aminobutanoate + 2-oxoglutarate | - |
Saccharomyces cerevisiae | 4-oxobutanoate + L-glutamate | - |
? | |
4-aminobutanoate + 2-oxoglutarate | - |
Schizosaccharomyces pombe | 4-oxobutanoate + L-glutamate | - |
? | |
4-aminobutanoate + 2-oxoglutarate | - |
Lachancea kluyveri | 4-oxobutanoate + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | - |
Saccharomyces cerevisiae | malonic semialdehyde + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | - |
Schizosaccharomyces pombe | malonic semialdehyde + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | - |
Lachancea kluyveri | malonic semialdehyde + L-glutamate | - |
? | |
additional information | does not use pyruvate as amino acceptor | Lachancea kluyveri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, SDS-PAGE | Lachancea kluyveri |
Synonyms | Comment | Organism |
---|---|---|
gamma-aminobutyrate aminotransferase | - |
Lachancea kluyveri |
SkUga1p | - |
Lachancea kluyveri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Lachancea kluyveri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | more than 70% of maximum activity | Lachancea kluyveri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Lachancea kluyveri |