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Literature summary for 2.6.1.19 extracted from

  • Yonaha, K.; Toyama, S.
    gamma-Aminobutyrate:alpha-ketoglutarate aminotransferase from Pseudomonas sp. F-126: purification, crystallization, and enzymologic properties (1980), Arch. Biochem. Biophys., 200, 156-164.
    View publication on PubMed
Search for more literature for 2.6.1.19

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas sp.

General Stability

General Stability Organism
pyridoxal phosphate protects against heat inactivation Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
3-Methyl-2-benzothiazolone hydrazone hydrochloride
-
 Pseudomonas sp.
Aminooxyacetate
-
 Pseudomonas sp.
carbonyl reagents
-
 Pseudomonas sp.
Cu2+
-
 Pseudomonas sp.
D-cycloserine
-
 Pseudomonas sp.
D-penicillamine
-
 Pseudomonas sp.
DL-cysteine
-
 Pseudomonas sp.
gabaculine
-
 Pseudomonas sp.
HgCl2
-
 Pseudomonas sp.
hydrazine
-
 Pseudomonas sp.
hydroxylamine
-
 Pseudomonas sp.
monoiodoacetate not Pseudomonas sp.
additional information no inhibition by chelating agents, non-substrate L- or D-amino acids, metal ions Pseudomonas sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.
phenylhydrazine
-
 Pseudomonas sp.
SH-group reagents 2-mercaptoethanol or DTT reactivates Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 2-oxoglutarate pH 8.0, 37°C Pseudomonas sp.
3.3 4.8 4-aminobutanoate pH 8.0, 37°C Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
176000 178000 gel filtration, sedimentation equilibrium centrifugation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate Pseudomonas sp. key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass 4-oxobutanoate + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate Pseudomonas sp. F-126 key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass 4-oxobutanoate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 F-126
-
Pseudomonas sp. F-126
-
 F-126
-

Purification (Commentary)

Purification (Comment) Organism
F-126 Pseudomonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
after inhibition by carbonyl reagents or sulfhydryl reagents enzyme may be reactivated by incubation with pyridoxal 5-phosphate or thiol reagents Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.8
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate
-
 Pseudomonas sp. 4-oxobutanoate + L-glutamate
-
 r
4-aminobutanoate + 2-oxoglutarate key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass Pseudomonas sp. 4-oxobutanoate + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate
-
 Pseudomonas sp. F-126 4-oxobutanoate + L-glutamate
-
 r
4-aminobutanoate + 2-oxoglutarate key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass Pseudomonas sp. F-126 4-oxobutanoate + L-glutamate
-
 ?
4-aminobutanoate + glyoxylate at 16% of the activity of 2-oxoglutarate Pseudomonas sp. 4-oxobutanoate + glycine
-
 r
4-aminobutanoate + glyoxylate at 16% of the activity of 2-oxoglutarate Pseudomonas sp. F-126 4-oxobutanoate + glycine
-
 r
4-aminobutanoate + pyruvate at 7% of the activity of 2-oxoglutarate Pseudomonas sp. 4-oxobutanoate + alanine
-
 r
4-aminobutanoate + pyruvate at 7% of the activity of 2-oxoglutarate Pseudomonas sp. F-126 4-oxobutanoate + alanine
-
 r
cadaverine + 2-oxoglutarate poor amino donor Pseudomonas sp. L-glutamate + ?
-
 r
D-lysine + 2-oxoglutarate poor amino donor Pseudomonas sp. L-glutamate + 5-aminopentanal
-
 r
DL-ornithine + 2-oxoglutarate poor amino donor Pseudomonas sp. L-glutamate + 4-methyl-2-oxopentanoate
-
 ?
hypotaurine + 2-oxoglutarate poor amino donor Pseudomonas sp. L-glutamate + 2-oxoethanesulfinic acid
-
 r
additional information no substrates are taurine, 3-aminopropane sulfonate, glycine Pseudomonas sp. ?
-
 ?
additional information no substrates are taurine, 3-aminopropane sulfonate, glycine Pseudomonas sp. F-126 ?
-
 ?
putrescine + 2-oxoglutarate poor amino donor Pseudomonas sp. L-glutamate + 4-aminobutanal
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 linear increase, above 65°C rapid decrease Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 pyridoxal phosphate protects against heat inactivation Pseudomonas sp.
37
-
 15 min stable, pH 6-10 Pseudomonas sp.
60
-
 up to, at least 15 min stable, pH 8 Pseudomonas sp.
65
-
 above, 15 min, inactivation Pseudomonas sp.
70
-
 15 min stable in the presence of pyridoxal phosphate Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11 no activity above or below Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 15 min stable, 37°C Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas sp.