Cloned (Comment) | Organism |
---|---|
gene glmS, screening of mutant library, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A594G | site-directed mutagenesis, the mutant shows unaltered activity (5 U/l) compared to wild-type (5 U/l) | Bacillus subtilis |
K595H | site-directed mutagenesis, the mutant shows unaltered activity (5 U/l) compared to wild-type (5 U/l) | Bacillus subtilis |
L593S | site-directed mutagenesis, the mutant shows highly increased activity (48 U/l) compared to wild-type (5 U/l) | Bacillus subtilis |
additional information | improvement of enzyme BsGlms activity through computer simulation and site saturation mutagenesis, method, overview | Bacillus subtilis |
S596F | site-directed mutagenesis, the mutant shows slightly increased activity (7 U/l) compared to wild-type (5 U/l) | Bacillus subtilis |
V597R | site-directed mutagenesis, the mutant shows slightly increased activity (8 U/l) compared to wild-type (5 U/l) | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding energy calculations of wild-type and mutant enzymes | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | Bacillus subtilis | - |
L-glutamate + D-glucosamine 6-phosphate | - |
? | |
L-glutamine + D-fructose 6-phosphate | Bacillus subtilis 168 | - |
L-glutamate + D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P0CI73 | - |
- |
Bacillus subtilis 168 | P0CI73 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Bacillus subtilis | L-glutamate + D-glucosamine 6-phosphate | - |
? | |
L-glutamine + D-fructose 6-phosphate | - |
Bacillus subtilis 168 | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BsglmS | - |
Bacillus subtilis |
GlmS | - |
Bacillus subtilis |
glucosamine-6-phosphate synthase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
additional information | mutation of residue Ser593 enhances the flexibility of the protein, which ultimately leads to increased enzyme activity, molecular dynamics simulation, homology modeling, and protein-ligand docking, overview | Bacillus subtilis |