Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | three recombinant versions containing internal oligoHis fragments are constructed: (a) by substituting residues 343-348 of the interdomain undecapeptide linker with hexaHis, (b) by replacing solvent-exposed residues 655-660 of the isomerase domain with hexaHis, and (c) by replacing amino acids at positions 568 and 569 with His residues to generate the three-dimensional hexaHis microdomain in the enzyme quaternary structure. The resulting constructs are purified to homogeneity. Catalytic properties are comparable with that of the wild-type enzyme. The construct containing the 655-660 hexaHis insert is found to be a homodimeric protein | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
uridine 5'-diphospho-N-acetyl-D-glucosamine | - |
Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.12 | - |
D-fructose 6-phosphate | mutant mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C | Candida albicans | |
1.33 | - |
L-glutamate | mutant Gfa1-K568H/S569H, pH 6.8, 37°C | Candida albicans | |
1.36 | - |
L-glutamate | mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C | Candida albicans | |
1.41 | - |
L-glutamate | wild-type, pH 6.8, 37°C | Candida albicans | |
1.56 | - |
D-fructose 6-phosphate | wild-type, pH 6.8, 37°C | Candida albicans | |
1.9 | - |
D-fructose 6-phosphate | mutant mutant Gfa1-K568H/S569H, pH 6.8, 37°C | Candida albicans | |
2.36 | - |
L-glutamate | mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C | Candida albicans | |
2.6 | - |
D-fructose 6-phosphate | mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
SDS-PAGE, wild-type and mutants | Candida albicans |
160000 | - |
gel filtration, mutant Gfa1-His6DELTA655-660 | Candida albicans |
328000 | - |
gel filtration, mutant Gfa1-K568H/S569H | Candida albicans |
335000 | - |
gel filtration, mutant Gfa1-His6DELTA343-348 | Candida albicans |
340000 | - |
gel filtration, wildtype | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using one-step immobilized metal-ion affinity chromatography | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Candida albicans | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucosamine-6-phosphate synthase | - |
Candida albicans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.55 | - |
wild-type, pH 6.8, 37°C | Candida albicans | uridine 5'-diphospho-N-acetyl-D-glucosamine | |
1.7 | - |
mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C | Candida albicans | uridine 5'-diphospho-N-acetyl-D-glucosamine | |
1.85 | - |
mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C | Candida albicans | uridine 5'-diphospho-N-acetyl-D-glucosamine | |
2 | - |
mutant Gfa1-K568H/S569H, pH 6.8, 37°C | Candida albicans | uridine 5'-diphospho-N-acetyl-D-glucosamine |