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Literature summary for 2.6.1.16 extracted from

  • Jedrzejczak, R.; Wojciechowski, M.; Andruszkiewicz, R.; Sowieski, P.; Kot-Wasik, A.; Milewski, S.
    Inactivation of glucosamine-6-phosphate synthase by N3-oxoacyl derivatives of L-2,3-diaminopropanoic acid (2012), ChemBioChem, 13, 85-96.
    View publication on PubMed
Search for more literature for 2.6.1.16

Inhibitors

Inhibitors Comment Organism Structure
(2S)-2-amino-3-(([(2R,3R)-3-benzoyloxiran-2-yl]carbonyl)amino)propanoic acid
-
 Saccharomyces cerevisiae
(2S)-2-amino-3-([(2E)-4-oxo-4-phenylbut-2-enoyl]amino)propanoic acid
-
 Saccharomyces cerevisiae
(2S)-2-amino-3-([(2E)-4-oxopent-2-enoyl]amino)propanoic acid
-
 Saccharomyces cerevisiae
(2S)-3-(([(2R,3R)-3-acetyloxiran-2-yl]carbonyl)amino)-2-aminopropanoic acid
-
 Saccharomyces cerevisiae
UDP-N-acetyl-alpha-D-glucosamine
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 D-fructose 6-phosphate pH 7.0, 37°C Saccharomyces cerevisiae
0.74
-
 L-glutamine pH 7.0, 37°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 4 * 80000 Saccharomyces cerevisiae
320000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme is purified to at least 98% homogeneity with 42% final yield Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + D-fructose 6-phosphate
-
 Saccharomyces cerevisiae L-glutamate + D-glucosamine 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 80000 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
glucosamine-6-phosphate synthase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.5
-
 L-glutamine co-substrate: D-fructose 6-phosphate, pH 7.0, 37°C Saccharomyces cerevisiae
35.5
-
 D-fructose 6-phosphate co-substrate: L-glutamine, pH 7.0, 37°C Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.056
-
 (2S)-2-amino-3-([(2E)-4-oxo-4-phenylbut-2-enoyl]amino)propanoic acid pH 7.0, 37°C Saccharomyces cerevisiae
0.45
-
 (2S)-2-amino-3-(([(2R,3R)-3-benzoyloxiran-2-yl]carbonyl)amino)propanoic acid pH 7.0, 37°C Saccharomyces cerevisiae
15.2
-
 (2S)-2-amino-3-([(2E)-4-oxopent-2-enoyl]amino)propanoic acid pH 7.0, 37°C Saccharomyces cerevisiae
19.4
-
 (2S)-3-(([(2R,3R)-3-acetyloxiran-2-yl]carbonyl)amino)-2-aminopropanoic acid pH 7.0, 37°C Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.27
-
 pH 7.0, 37°C Saccharomyces cerevisiae (2S)-2-amino-3-([(2E)-4-oxo-4-phenylbut-2-enoyl]amino)propanoic acid
0.52
-
 pH 7.0, 37°C Saccharomyces cerevisiae UDP-N-acetyl-alpha-D-glucosamine
0.66
-
 pH 7.0, 37°C Saccharomyces cerevisiae (2S)-2-amino-3-(([(2R,3R)-3-benzoyloxiran-2-yl]carbonyl)amino)propanoic acid
5.6
-
 pH 7.0, 37°C Saccharomyces cerevisiae (2S)-2-amino-3-([(2E)-4-oxopent-2-enoyl]amino)propanoic acid
7.3
-
 pH 7.0, 37°C Saccharomyces cerevisiae (2S)-3-(([(2R,3R)-3-acetyloxiran-2-yl]carbonyl)amino)-2-aminopropanoic acid