General Stability | Organism |
---|---|
2-mercaptoethanol and alpha-keto acids stabilize | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Mercaptopyruvate | - |
Rattus norvegicus | |
4-methylsulfanyl-2-oxobutanoate | - |
Rattus norvegicus | |
carbonyl reagents | - |
Rattus norvegicus | |
hydroxylamine | - |
Rattus norvegicus | |
Isonicotinic acid hydrazide | - |
Rattus norvegicus | |
Sodium borohydride | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
L-methionine | pH 8.4, 37ºC | Rattus norvegicus | |
2 | - |
L-glutamine | pH 8.4, 37ºC | Rattus norvegicus | |
2 | - |
L-methionine (SR)-sulfoxide | pH 8.4, 37ºC | Rattus norvegicus | |
3.2 | - |
4-methylsulfanyl-2-oxobutanoate | pH 8.4, 37ºC | Rattus norvegicus | |
4.4 | - |
L-glutamic acid gamma-methylester | pH 8.4, 37ºC | Rattus norvegicus | |
4.8 | - |
3-Mercaptopyruvate | pH 8.4, 37ºC | Rattus norvegicus | |
8 | - |
glyoxylate | pH 8.4, 37ºC | Rattus norvegicus | |
8 | - |
3-hydroxypyruvate | pH 8.4, 37ºC | Rattus norvegicus | |
10.5 | - |
pyruvate | pH 8.4, 37ºC | Rattus norvegicus | |
50 | - |
2-Oxoglutaramate | pH 8.4, 37ºC | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + alpha-keto acid | Rattus norvegicus | - |
alpha-ketoglutarate + amino acid | - |
? | |
L-glutamine + pyruvate | Rattus norvegicus | - |
2-oxoglutaramate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Sprague-Dawley | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation , DE-52 chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, at least 7 weeks | Rattus norvegicus |
0-5°C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, little or no loss of activity for 1-2 weeks, in the absence of mercaptoethanol inactivation within 1-2 days | Rattus norvegicus |
24-26°C, 20% loss of activity of purified enzyme in 3 days | Rattus norvegicus |
4ºC, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutaramate + glycine | poor substrate | Rattus norvegicus | L-glutamine + glyoxylate | - |
? | |
2-oxoglutaramate + L-alanine | poor substrate | Rattus norvegicus | L-glutamine + pyruvate | - |
? | |
2-oxoglutaramate + L-asparagine | poor substrate | Rattus norvegicus | L-glutamine + 2-oxosuccinamate | - |
? | |
2-oxoglutaramate + L-cysteine | poor substrate | Rattus norvegicus | L-glutamine + 3-mercapto-2-oxopropanoate | - |
? | |
2-oxoglutaramate + L-ethionine | - |
Rattus norvegicus | L-glutamine + 4-ethylsulfanyl-2-oxobutanoate | - |
? | |
2-oxoglutaramate + L-glutamic acid 4-benzylester | poor substrate | Rattus norvegicus | L-glutamine + 2-oxopentane-1,5-dioic acid benzyl ester | - |
? | |
2-oxoglutaramate + L-homoserine | - |
Rattus norvegicus | L-glutamine + 4-hydroxy-2-oxobutanoate | - |
? | |
2-oxoglutaramate + L-methionine | best amino group donor | Rattus norvegicus | L-glutamine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
2-oxoglutaramate + L-methionine (SR)-sulfoxide | - |
Rattus norvegicus | L-glutamine + 4-methylsulfinyl-2-oxobutanoate | - |
? | |
2-oxoglutaramate + L-methionine sulfone | - |
Rattus norvegicus | L-glutamine + 4-methylsulfonyl-2-oxobutanoate | - |
? | |
2-oxoglutaramate + L-phenylalanine | poor substrate | Rattus norvegicus | L-glutamine + phenylpyruvate | - |
? | |
2-oxoglutaramate + L-serine | poor substrate | Rattus norvegicus | L-glutamine + 3-hydroxy-2-oxopropanoate | - |
? | |
glyoxylate + 4-glutamylmethylamide | - |
Rattus norvegicus | glycine + ? | - |
? | |
glyoxylate + DL-homocysteine | poor substrate | Rattus norvegicus | glycine + 2-oxo-4-thiobutanoate | - |
? | |
glyoxylate + glutamic acid 4-methyl ester | - |
Rattus norvegicus | glycine + 2-oxoglutarate 4-methyl ester | - |
? | |
glyoxylate + L-2-amino-4-oxo-5-chloropentanoic acid | poor substrate | Rattus norvegicus | glycine + 5-chloro-2,4-dioxopentanoate | - |
? | |
glyoxylate + L-alanine | poor substrate | Rattus norvegicus | glycine + pyruvate | - |
? | |
glyoxylate + L-asparagine | poor substrate | Rattus norvegicus | glycine + 2-oxosuccinamate | - |
? | |
glyoxylate + L-cysteine | poor substrate | Rattus norvegicus | glycine + 3-mercapto-2-oxopropanoate | - |
? | |
glyoxylate + L-ethionine | - |
Rattus norvegicus | glycine + 4-ethylsulfanyl-2-oxobutanoate | - |
? | |
glyoxylate + L-glutamine | - |
Rattus norvegicus | glycine + 2-oxoglutaramate | - |
? | |
glyoxylate + L-homoserine | poor substrate | Rattus norvegicus | glycine + 4-hydroxy-2-oxobutanoate | - |
? | |
glyoxylate + L-methionine | - |
Rattus norvegicus | glycine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
glyoxylate + L-methionine (SR)-sulfoxide | - |
Rattus norvegicus | glycine + ? | - |
? | |
glyoxylate + L-methionine sulfone | - |
Rattus norvegicus | glycine + 4-methylsulfonyl-2-oxobutanoate | - |
? | |
glyoxylate + L-phenylalanine | poor substrate | Rattus norvegicus | glycine + phenylpyruvate | - |
? | |
glyoxylate + L-serine | poor substrate | Rattus norvegicus | glycine + 3-hydroxy-2-oxopropanoate | - |
? | |
glyoxylate + S-methyl-L-cysteine | poor substrate | Rattus norvegicus | glycine + 3-methylsulfanyl-2-oxopropanoate | - |
? | |
L-glutamine + 2-oxoglutaramate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamine | - |
? | |
L-glutamine + 3-hydroxypyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-serine | - |
? | |
L-glutamine + 3-mercaptopyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-cysteine | - |
? | |
L-glutamine + 4-methylsulfanyl-2-oxobutanoate | - |
Rattus norvegicus | 2-oxoglutaramate + L-methionine | - |
? | |
L-glutamine + alpha-keto acid | - |
Rattus norvegicus | alpha-ketoglutarate + amino acid | - |
? | |
L-glutamine + pyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-alanine | - |
? | |
pyruvate + L-4-glutamylmethylamide | - |
Rattus norvegicus | L-alanine + ? | - |
? | |
pyruvate + L-glutamine | L-glutamine cannot be replaced by glycine | Rattus norvegicus | L-alanine + 2-oxoglutaramate | - |
? | |
pyruvate + L-methionine | poor substrate | Rattus norvegicus | L-alanine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
pyruvate + L-methionine (SR)-sulfoxide | poor substrate | Rattus norvegicus | L-alanine + 4-methylsulfinyl-2-oxobutanoate | - |
? | |
pyruvate + L-methionine sulfone | poor substrate | Rattus norvegicus | L-alanine + 4-methylsulfonyl-2-oxobutanoate | - |
? | |
pyruvate + L-phenylalanine | - |
Rattus norvegicus | L-alanine + phenylpyruvate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
in crude extract inactivation within 10 min, in the presence of pyruvate, glyoxylate or phenylpyruvate 1 h stability, 25% loss of activity within 3 h | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.8 | about half-maximum activity at pH 7.6 and about 90% of maximum activity at pH 8.8 | Rattus norvegicus |