Crystallization (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminobutanoate | - |
Rattus norvegicus | |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Rattus norvegicus | |
5-Aminopentanoate | - |
Rattus norvegicus | |
Butanoate | - |
Rattus norvegicus | |
Butyrate | 27% inhibition at 25 mM | Rattus norvegicus | |
caproate | 34% inhibition at 25 mM | Rattus norvegicus | |
DL-norvaline | 44% inhibition at 100 mM | Rattus norvegicus | |
hexanoate | - |
Rattus norvegicus | |
iodoacetamide | - |
Rattus norvegicus | |
Isocaproate | 41% inhibition at 25 mM | Rattus norvegicus | |
Isohexanoate | - |
Rattus norvegicus | |
Isopentanoate | - |
Rattus norvegicus | |
Isovalerate | 41% inhibition at 25 mM | Rattus norvegicus | |
L-canavanine | 23% inhibition at 20 mM | Rattus norvegicus | |
L-isoleucine | 19% inhibition at 50 mM | Rattus norvegicus | |
L-leucine | 17% inhibition at 50 mM | Rattus norvegicus | |
L-valine | 33% inhibition at 50 mM | Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus | |
valerate | 38% inhibition at 25 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for ornithine increases below pH 7.5, Km for 2-oxoglutarate decreases below pH 7 | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Rattus norvegicus |
161000 | - |
equilibrium sedimentation analysis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-15°C, pH 6-8, 0.01 M potassium phosphate buffer, about 20% loss of activity after 4 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | - |
Rattus norvegicus | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
pH 7.5, 15 min, 85% loss of activity, 60 min, 96% loss of activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | equally stable at pH 6.8 and pH 8.0 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 4 mol of pyridoxal phosphate per mol of enzyme | Rattus norvegicus |