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Literature summary for 2.6.1.13 extracted from
- Crystallization and properties of human liver ornithine aminotransferase (1982), J. Biochem., 92, 1785-1792.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
2-oxoglutarate | pH 8, 37°C | Rattus norvegicus |  |
| 1.1 | - |
L-ornithine | pH 8, 37°C | Rattus norvegicus | |
| 1.8 | - |
L-ornithine | pH 8, 37°C | Homo sapiens | |
| 2.7 | - |
2-oxoglutarate | pH 8, 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
4 * 44000, SDS-PAGE | Homo sapiens |
| 177000 | - |
sucrose density gradient centrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| lymphoblast | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Rattus norvegicus |
| 33 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM potassium phosphate buffer, pH 8, 5 mM 2-oxoglutarate, 0.02 mM pyridoxal phosphate, 90-100% retention of activity after 1 month | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine + 2-oxoglutarate | - |
Homo sapiens | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
| L-ornithine + 2-oxoglutarate | - |
Rattus norvegicus | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Rattus norvegicus |
| tetramer | 4 * 44000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
| 8 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 50% of activity maximum, pH 9.0: about 60% of activity maximum | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
| pyridoxal 5'-phosphate | Km: 0.0018 mM | Homo sapiens | |
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