Cloned (Comment) | Organism |
---|---|
gene neo-6, DNA and amino acid sequence determination and analysis, overexpression as His6-tagged enzyme in Esccherichia coli | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
46801 | - |
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
46951 | - |
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae NCIMB 8233 | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Streptomyces fradiae | Q53U20 | gene neo-6 encoded in the neo gene cluster | - |
Streptomyces fradiae NCIMB 8233 | - |
- |
- |
Streptomyces fradiae NCIMB 8233 | Q53U20 | gene neo-6 encoded in the neo gene cluster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae NCIMB 8233 | 2-oxoglutaramate + 2-deoxystreptamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
Synonyms | Comment | Organism |
---|---|---|
btrR | - |
Streptomyces fradiae |
BtrS | - |
Streptomyces fradiae |
L-glutamine: 2-deoxy-scyllo-inosamine aminotransferase | - |
Streptomyces fradiae |
L-glutamine:DOI aminotransferase | - |
Streptomyces fradiae |
neo-6 | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity | Streptomyces fradiae |
General Information | Comment | Organism |
---|---|---|
evolution | BtrR and StsC are the two homologues of Neo-6 in butirosin and streptamycin biosynthetic pathways | Streptomyces fradiae |
metabolism | purified Neo-6 also catalyses the amino transfer from L-glutamine to 2-deoxyscyllo-inosose to produce 2-deoxyscyllo-inosamine when coupled with BtrC in the presence of G6P, NAD+, Co2+ and L-glutamine, EC 2.6.1.100 | Streptomyces fradiae |
additional information | gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview | Streptomyces fradiae |
physiological function | the enzyme produces 2-deoxystreptamine (DOS), which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis, function of Neo-6 as the L-glutamine:DOI aminotransferase | Streptomyces fradiae |