Cloned (Comment) | Organism |
---|---|
gene btrR, recombinant expression of His-tagged BtrR in Escherichia coli strain BL 21 (DE3) | Niallia circulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
50703 | - |
2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
50714 | - |
2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
97000 | - |
recombinant His-tagged enzyme, gel filtration | Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-deoxy-scyllo-inosose | Niallia circulans | - |
2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
L-glutamine + 2-deoxy-scyllo-inosose | Niallia circulans NRRL B3312 | - |
2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q8G8Y2 | gene btrR encoded in the butirosin cluster | - |
Niallia circulans NRRL B3312 | Q8G8Y2 | gene btrR encoded in the butirosin cluster | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged BtrR from Escherichia coli strain BL 21 (DE3) by cobalt affinity chromatography, gel filtration, and anion exchange chromatography | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-deoxy-scyllo-inosose | - |
Niallia circulans | 2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
L-glutamine + 2-deoxy-scyllo-inosose | - |
Niallia circulans NRRL B3312 | 2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
Synonyms | Comment | Organism |
---|---|---|
btrR | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Niallia circulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Niallia circulans |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses the transamination of 2-deoxy-scyllo-inosose to give 2-deoxy-scyllo-inosamine, an intermediate in the biosynthesis of 2-deoxystreptamine, biosynthetic pathway of 2-deoxystreptamine, overview | Niallia circulans |
physiological function | BtrR catalyzes the synthesis of 2-deoxystreptamine, which is the common aglycone of a class of aminoglycoside antibiotics, of which many are clinically important aminoglycosides like kanamycin, neomycin, gentamicin and butirosin | Niallia circulans |