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Literature summary for 2.6.1.100 extracted from

  • Kudo, F.; Yamamoto, Y.; Yokoyama, K.; Eguchi, T.; Kakinuma, K.
    Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773 (2005), J. Antibiot., 58, 766-774.
    View publication on PubMed
Search for more literature for 2.6.1.100

Cloned(Commentary)

Cloned (Comment) Organism
gene neoB, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli Streptomyces fradiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae
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 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae NBRC 12773
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 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae Q53U20 gene neoB, encoded in the neomycin biosynthetic gene cluster
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Streptomyces fradiae NBRC 12773 Q53U20 gene neoB, encoded in the neomycin biosynthetic gene cluster
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptomyces fradiae 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptomyces fradiae NBRC 12773 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
additional information NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 Streptomyces fradiae ?
-
 ?
additional information NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 Streptomyces fradiae NBRC 12773 ?
-
 ?

Synonyms

Synonyms Comment Organism
Neo6
-
 Streptomyces fradiae
neoB
-
 Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Streptomyces fradiae

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 Streptomyces fradiae
additional information the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS Streptomyces fradiae