Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | competitive to L-aspartate | Methanothermobacter thermautotrophicus | |
adipate | moderately | Methanothermobacter thermautotrophicus | |
fumarate | weak | Methanothermobacter thermautotrophicus | |
Glutarate | weak | Methanothermobacter thermautotrophicus | |
Maleate | - |
Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Methanothermobacter thermautotrophicus |
57000 | - |
gel filtration, gradient PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Methanothermobacter thermautotrophicus | - |
oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | Methanothermobacter thermautotrophicus SF-4 | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus SF-4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.44 | - |
purified enzyme | Methanothermobacter thermautotrophicus |
Storage Stability | Organism |
---|---|
-20°C or 4°C, stable for at least 2 weeks | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | 25.2% of the activity with glutamate | Methanothermobacter thermautotrophicus | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus SF-4 | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | 25.2% of the activity with glutamate | Methanothermobacter thermautotrophicus SF-4 | oxaloacetate + L-glutamate | - |
? | |
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
? | |
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus SF-4 | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
? | |
L-glutamate + 2-oxoglutarate | L-glutamate is best amino acid donor | Methanothermobacter thermautotrophicus | 2-oxoglutarate + L-glutamate | - |
? | |
L-glutamate + 2-oxoglutarate | L-glutamate is best amino acid donor | Methanothermobacter thermautotrophicus SF-4 | 2-oxoglutarate + L-glutamate | - |
? | |
L-methionine + 2-oxoglutarate | 1.0% of the activity with glutamate | Methanothermobacter thermautotrophicus | L-glutamate + 4-methylsulfanyl-2-oxobutyric acid | - |
? | |
additional information | substrate specificity | Methanothermobacter thermautotrophicus | ? | - |
? | |
additional information | substrate specificity | Methanothermobacter thermautotrophicus SF-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
pH 7.5, 1 h, stable up to | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Methanothermobacter thermautotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | - |
Methanothermobacter thermautotrophicus |