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Literature summary for 2.5.1.B31 extracted from

  • Ozaki, T.; Nishiyama, M.; Kuzuyama, T.
    Novel tryptophan metabolism by a potential gene cluster that is widely distributed among Actinomycetes (2013), J. Biol. Chem., 288, 9946-9956.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene SCO7468, genetic organization and sequence comparison, functional recombinant expression in Streptomyces lividans strain TK23, the overexpression of 5-DMATSSc results in 5-dimethylallylindole-3-acetonitrile overproduction in the transformant. The heterologous host Streptomyces lividans TK23 possesses a gene cluster almost identical to that of Streptomyces coelicolor A3(2). Recombinant expression in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA at 5 mM Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic analysis demonstrates that the SCO7467 reaction follows Michaelis-Menten kinetics Streptomyces coelicolor
0.05
-
dimethylallyl diphosphate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
0.08
-
L-tryptophan pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the SCO7467-catalyzed prenylation reaction is Mg2+-independent Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan Streptomyces coelicolor
-
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan Streptomyces coelicolor A3(2)
-
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9ADJ2 gene SCO7467
-
Streptomyces coelicolor A3(2) Q9ADJ2 gene SCO7467
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from in Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan
-
Streptomyces coelicolor diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan
-
Streptomyces coelicolor diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan product identification by NMR and mass spectroscopy ?
dimethylallyl diphosphate + L-tryptophan
-
Streptomyces coelicolor A3(2) diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan
-
Streptomyces coelicolor A3(2) diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan product identification by NMR and mass spectroscopy ?

Synonyms

Synonyms Comment Organism
5-DMATSSc
-
Streptomyces coelicolor
SCO7467
-
Streptomyces coelicolor
tryptophan C5-prenyltransferase
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
L-tryptophan pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor
0.4
-
dimethylallyl diphosphate pH 8.0, 30°C, recombinant enzyme Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptomyces coelicolor