Cloned (Comment) | Organism |
---|---|
gene SCO7468, genetic organization and sequence comparison, functional recombinant expression in Streptomyces lividans strain TK23, the overexpression of 5-DMATSSc results in 5-dimethylallylindole-3-acetonitrile overproduction in the transformant. The heterologous host Streptomyces lividans TK23 possesses a gene cluster almost identical to that of Streptomyces coelicolor A3(2). Recombinant expression in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA at 5 mM | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis demonstrates that the SCO7467 reaction follows Michaelis-Menten kinetics | Streptomyces coelicolor | |
0.05 | - |
dimethylallyl diphosphate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
0.08 | - |
L-tryptophan | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the SCO7467-catalyzed prenylation reaction is Mg2+-independent | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | Streptomyces coelicolor | - |
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | Streptomyces coelicolor A3(2) | - |
diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9ADJ2 | gene SCO7467 | - |
Streptomyces coelicolor A3(2) | Q9ADJ2 | gene SCO7467 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from in Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | - |
Streptomyces coelicolor | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Streptomyces coelicolor | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | product identification by NMR and mass spectroscopy | ? | |
dimethylallyl diphosphate + L-tryptophan | - |
Streptomyces coelicolor A3(2) | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Streptomyces coelicolor A3(2) | diphosphate + 5-(3-methylbut-2-enyl)-L-tryptophan | product identification by NMR and mass spectroscopy | ? |
Synonyms | Comment | Organism |
---|---|---|
5-DMATSSc | - |
Streptomyces coelicolor |
SCO7467 | - |
Streptomyces coelicolor |
tryptophan C5-prenyltransferase | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-tryptophan | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
0.4 | - |
dimethylallyl diphosphate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces coelicolor |