Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His-tagged LePGT1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method | Lithospermum erythrorhizon |
Protein Variants | Comment | Organism |
---|---|---|
D212A | site-directed mutagenesis, the mutant shows almost no expression of the recombinant protein | Lithospermum erythrorhizon |
D87A | site-directed mutagenesis, the mutant can be be successfully expressed, but is catalytically inactive | Lithospermum erythrorhizon |
additional information | the substitution of aspartate with alanine, especially D87 and D212, causes a dramatic decrease in enzyme activity | Lithospermum erythrorhizon |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxybenzoic acid | 10% inhibition | Lithospermum erythrorhizon | |
caffeic acid | 30% inhibition | Lithospermum erythrorhizon | |
catechol | 89% inhibition | Lithospermum erythrorhizon | |
chlorogenic acid | 20% inhibition | Lithospermum erythrorhizon | |
homogentisic acid | 93% inhibition | Lithospermum erythrorhizon | |
hydroquinone | 98% inhibition | Lithospermum erythrorhizon | |
additional information | compounds showing strong competition/inhibition are simple phenols having phenolic OH residues, while carboxylic acid does not seem to influence the enzyme activity. Phenylpropanoid gives no apparent inhibition. No or poor inhibition by benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, ferulic acid, and 4-coumaric acid | Lithospermum erythrorhizon |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Lithospermum erythrorhizon | |
0.0138 | - |
geranyl diphosphate | recombinant enzyme, pH 7.0-7.5, 45°C | Lithospermum erythrorhizon | |
0.0184 | - |
4-hydroxybenzoate | recombinant enzyme, pH 7.0-7.5, 45°C | Lithospermum erythrorhizon |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Lithospermum erythrorhizon | 16020 | - |
microsome | - |
Lithospermum erythrorhizon | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | results in 34.8% of the activity with Mg2+ | Lithospermum erythrorhizon | |
Fe2+ | results in 15.0% of the activity with Mg2+ | Lithospermum erythrorhizon | |
Mg2+ | preferred divalent cation, required | Lithospermum erythrorhizon | |
Mn2+ | results in 8.54% of the activity with Mg2+ | Lithospermum erythrorhizon | |
additional information | no activity with Ca2+, Cu2+, and Zn2+ | Lithospermum erythrorhizon |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Lithospermum erythrorhizon |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 4-hydroxybenzoate | Lithospermum erythrorhizon | - |
3-geranyl-4-hydroxybenzoate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithospermum erythrorhizon | Q8W405 | gene PGT-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged LePGT1 41.3fold from Spodoptera frugiperda Sf9 cells by improved detergent solubilization with 6 mM sodium deoxycholate, and nickel affinity chromatography | Lithospermum erythrorhizon |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0665 | - |
recombinant enzyme in crude Sf9 cell extract, pH 7.0-7.5, 45°C | Lithospermum erythrorhizon |
4.68 | - |
purified recombinant enzyme, pH 7.0-7.5, 45°C | Lithospermum erythrorhizon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 4-hydroxybenzoate | - |
Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? | |
geranyl diphosphate + 4-hydroxybenzoate | LePGT1 reveals high specificity for PHB to give 3-geranyl-4-hydroxybenzoic acid (100%) | Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Lithospermum erythrorhizon |
More | the enzyme contains contains multiple transmembrane alpha-helices | Lithospermum erythrorhizon |
Synonyms | Comment | Organism |
---|---|---|
LePGT1 | - |
Lithospermum erythrorhizon |
p-hydroxybenzoate: geranyltransferase | - |
Lithospermum erythrorhizon |
PHB geranyltransferase | - |
Lithospermum erythrorhizon |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Lithospermum erythrorhizon |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | recombinant enzyme | Lithospermum erythrorhizon |
General Information | Comment | Organism |
---|---|---|
additional information | LePGT1 is a model membrane-bound aromatic substrate prenyltransferase | Lithospermum erythrorhizon |
physiological function | the enzyme is involved in the biosynthesis of a red naphthoquinone pigment, shikonin | Lithospermum erythrorhizon |