Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21trxB (DE3) from vector pET46 Ek/LIC | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, crystallization method screening, sitting drop vapour diffusion method, mixing of 0.002 ml of 3 mg/ml protein in 25 mM Tris-HCl, 150 mM NaCl, pH 7.5, with 0.002 ml of crystallization solution containing 0.3 M magnesium chloride hexahydrate, 0.1 M Tris-HCl, pH 8.5, 24% w/v PEG 3350, 3-4 days, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement, the crystal contains one homodimer per asymmetric unit, structure modelling | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Escherichia coli | the enzyme catalyzes the successive condensation of farnesyl diphosphate with five isopentenyl pyrophosphate molecules to form trans-C40-octaprenyl diphosphate | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AD57 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21trxB (DE3) by nickel affinity and anion exchange chromatography, dialysis, and ultrafiltration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | the enzyme catalyzes the successive condensation of farnesyl diphosphate with five isopentenyl pyrophosphate molecules to form trans-C40-octaprenyl diphosphate | Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
octaprenyl pyrophosphate synthase | - |
Escherichia coli |
Opp | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the E-type prenyltransferase family | Escherichia coli |
additional information | enzyme structure modelling using the structure of Rhodobacter capsulatus decaprenyl diphosphate synthase, PDB ID 3mzv, as a template model | Escherichia coli |
physiological function | the enzyme is essential for the biosynthesis of bacterial ubiquinone or menaquinone side chains, which play an important role in the electron-transport system | Escherichia coli |