Literature summary for 2.5.1.90 extracted from

Tonhosolo, R.; D'Alexandri, F.L.; Genta, F.A.; Wunderlich, G.; Gozzo, F.C.; Eberlin, M.N.; Peres, V.J.; Kimura, E.A.; Katzin, A.M.
Identification, molecular cloning and functional characterization of an octaprenyl pyrophosphate synthase in intra-erythrocytic stages of Plasmodium falciparum (2005), Biochem. J., 392, 117-126.

Search for more literature for 2.5.1.90

Cloned(Commentary)

Cloned (Comment)	Organism
expression as glutathione-S-transferase fusion protein	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
nerolidol	competitive, 57% inhibition at 50 nM	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0011	-	(2E,6E)-farnesyl diphosphate	pH 7.5, 30°C	Plasmodium falciparum
0.0024	-	isopentenyl diphosphate	pH 7.5, 30°C	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	x * 63000, SDS-PAGE of recombinant enzyme	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	O96130	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial, from schizont stage parasite	Plasmodium falciparum

Source Tissue

Source Tissue	Comment	Organism	Textmining
schizont	-	Plasmodium falciparum	-
trophozoite	-	Plasmodium falciparum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate	-	Plasmodium falciparum	5 diphosphate + all-trans-octaprenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate	-	Plasmodium falciparum	6 diphosphate + all-trans-nonaprenyl diphosphate	-	?
additional information	no substrate: geranyl diphosphate, geranylgeranyl diphosphate	Plasmodium falciparum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 63000, SDS-PAGE of recombinant enzyme	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
octaprenyl pyrophosphate synthase	-	Plasmodium falciparum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000015	-	nerolidol	pH 7.5, 30°C	Plasmodium falciparum

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Release 2023.1 (January 2023)