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Literature summary for 2.5.1.90 extracted from

  • Guo, R.T.; Kuo, C.J.; Chou, C.C.; Ko, T.P.; Shr, H.L.; Liang, P.H.; Wang, A.H.
    Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination (2004), J. Biol. Chem., 279, 4903-4912.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutants F52A, V73A, S77F, F132A, to 2.28, 2.80, 2.85, 2.45 and 2.40 A resolution, respectively. OPPs is composed entirely of alpha-helices joined by connecting loops and is arranged with nine core helices around a large central cavity. An elongated hydrophobic tunnel between D and F alpha-helices contains two DDXXD motifs on the top for substrate binding and is occupied at the bottom with two large residues Phe-52 and Phe-132 Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
A76Y main product is C20 Thermotoga maritima
A76Y/S77F main product is C20 Thermotoga maritima
F132A product is predominantly C50 Thermotoga maritima
F52A product is predominatly C40, like in wild-type Thermotoga maritima
S77F main product is C20 Thermotoga maritima
V73Y mutation leads to additional accumulation of C30 intermediate Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1M1
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
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Thermotoga maritima 5 diphosphate + all-trans-octaprenyl diphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00000023
-
(2E,6E)-farnesyl diphosphate mutant A76Y/S77F, pH 7.5, 25°C Thermotoga maritima
0.00000029
-
(2E,6E)-farnesyl diphosphate mutant S77F, pH 7.5, 25°C Thermotoga maritima
0.000073
-
(2E,6E)-farnesyl diphosphate mutant A76Y, pH 7.5, 25°C Thermotoga maritima
0.0033
-
(2E,6E)-farnesyl diphosphate mutant V37Y, pH 7.5, 25°C Thermotoga maritima
0.0035
-
(2E,6E)-farnesyl diphosphate mutant F52A, pH 7.5, 25°C Thermotoga maritima
0.005
-
(2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 25°C Thermotoga maritima
0.0051
-
(2E,6E)-farnesyl diphosphate mutant F132A, pH 7.5, 25°C Thermotoga maritima

General Information

General Information Comment Organism
physiological function the enzyme catalyzes consecutive condensation reactions of farnesyl diphosphate with isopentenyl diphosphate to generate octaprenyl diphosphate, which constitutes the side chain of bacterial ubiquinone or menaquinone Thermotoga maritima