Cloned (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutants F52A, V73A, S77F, F132A, to 2.28, 2.80, 2.85, 2.45 and 2.40 A resolution, respectively. OPPs is composed entirely of alpha-helices joined by connecting loops and is arranged with nine core helices around a large central cavity. An elongated hydrophobic tunnel between D and F alpha-helices contains two DDXXD motifs on the top for substrate binding and is occupied at the bottom with two large residues Phe-52 and Phe-132 | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
A76Y | main product is C20 | Thermotoga maritima |
A76Y/S77F | main product is C20 | Thermotoga maritima |
F132A | product is predominantly C50 | Thermotoga maritima |
F52A | product is predominatly C40, like in wild-type | Thermotoga maritima |
S77F | main product is C20 | Thermotoga maritima |
V73Y | mutation leads to additional accumulation of C30 intermediate | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X1M1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Thermotoga maritima | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000023 | - |
(2E,6E)-farnesyl diphosphate | mutant A76Y/S77F, pH 7.5, 25°C | Thermotoga maritima | |
0.00000029 | - |
(2E,6E)-farnesyl diphosphate | mutant S77F, pH 7.5, 25°C | Thermotoga maritima | |
0.000073 | - |
(2E,6E)-farnesyl diphosphate | mutant A76Y, pH 7.5, 25°C | Thermotoga maritima | |
0.0033 | - |
(2E,6E)-farnesyl diphosphate | mutant V37Y, pH 7.5, 25°C | Thermotoga maritima | |
0.0035 | - |
(2E,6E)-farnesyl diphosphate | mutant F52A, pH 7.5, 25°C | Thermotoga maritima | |
0.005 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH 7.5, 25°C | Thermotoga maritima | |
0.0051 | - |
(2E,6E)-farnesyl diphosphate | mutant F132A, pH 7.5, 25°C | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes consecutive condensation reactions of farnesyl diphosphate with isopentenyl diphosphate to generate octaprenyl diphosphate, which constitutes the side chain of bacterial ubiquinone or menaquinone | Thermotoga maritima |