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Literature summary for 2.5.1.9 extracted from

  • Fischer, M.; Romisch, W.; Illarionov, B.; Eisenreich, W.; Bacher, A.
    Structures and reaction mechanisms of riboflavin synthases of eubacterial and archaeal origin (2005), Biochem. Soc. Trans., 33, 780-784.
    View publication on PubMed
Search for more literature for 2.5.1.9

Protein Variants

Protein Variants Comment Organism
S41A site-directed mutagenesis, mutant produces a dimeric pentacyclic reaction intermediate, i.e. compound Q, which can be cleaved in 2 different ways by the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information stopped flow and quenched flow kinetics of wild-type enzyme and mutant S41A Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine Escherichia coli final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine Methanothermobacter thermautotrophicus final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine Arabidopsis thaliana final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine Schizosaccharomyces pombe final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine Methanocaldococcus jannaschii final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
additional information Methanothermobacter thermautotrophicus archaeal enzymes have diverged early in evolution from a common ancestor ?
-
 ?
additional information Methanocaldococcus jannaschii archaeal enzymes have diverged early in evolution from a common ancestor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Escherichia coli
-
-
-
Methanocaldococcus jannaschii
-
-
-
Methanothermobacter thermautotrophicus
-
-
-
Schizosaccharomyces pombe
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine catalytic mechanism, stereochemistry Methanothermobacter thermautotrophicus
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine catalytic mechanism, stereochemistry Arabidopsis thaliana
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine catalytic mechanism, stereochemistry Schizosaccharomyces pombe
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine catalytic mechanism, stereochemistry, pentacyclic reaction intermediate which is diastereomeric to the reaction intermediate of the enzyme from Methanococcus jannaschii Escherichia coli
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine catalytic mechanism, stereochemistry, pentacyclic reaction intermediate, which is diastereomeric to the reaction intermediate of the enzyme from Escherichia coli Methanocaldococcus jannaschii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
 substrate 6,7-dimethyl-8-(1-D-ribityl)lumazine Methanocaldococcus jannaschii
0.021
-
 with substrate compound Q, a dimer of the pentameric reaction intermediate Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview Escherichia coli riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview Methanothermobacter thermautotrophicus riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview Arabidopsis thaliana riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview Schizosaccharomyces pombe riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine final step of biosynthesis of vitamin B2, i.e. riboflavin, the universal precursor of flavocoenzymes, overview Methanocaldococcus jannaschii riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine dismutation, exchange of a C4 fragment, regio- and stereospecific reaction Escherichia coli riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine dismutation, exchange of a C4 fragment, regio- and stereospecific reaction Methanothermobacter thermautotrophicus riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine dismutation, exchange of a C4 fragment, regio- and stereospecific reaction Arabidopsis thaliana riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine dismutation, exchange of a C4 fragment, regio- and stereospecific reaction Schizosaccharomyces pombe riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
6,7-dimethyl-8-(1-D-ribityl)lumazine dismutation, exchange of a C4 fragment, regio- and stereospecific reaction Methanocaldococcus jannaschii riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
additional information archaeal enzymes have diverged early in evolution from a common ancestor Methanothermobacter thermautotrophicus ?
-
 ?
additional information archaeal enzymes have diverged early in evolution from a common ancestor Methanocaldococcus jannaschii ?
-
 ?
additional information active with the dimeric pentacyclic reaction intermediate as substrate Methanocaldococcus jannaschii ?
-
 ?
additional information no activity with the dimeric pentacyclic reaction intermediate as substrate Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
pentamer homopentamer, folding topology Methanothermobacter thermautotrophicus
pentamer homopentamer, folding topology Methanocaldococcus jannaschii
trimer 2 folding topologies, the active site can be formed at the subunit interface or each subunit binds one substrate molecule Arabidopsis thaliana
trimer folding topology, each subunit binds one substrate molecule Schizosaccharomyces pombe
trimer folding topology, the active site can be formed at the subunit interface Escherichia coli