Literature summary for 2.5.1.9 extracted from

Ladenstein, R.; Schneider, M.; Huber, R.; Bartunik, H.D.; Wilson, K.; Schott, K.; Bacher, A.
Heavy riboflavin synthase from Bacillus subtilis. Crystal structure analysis of the icosahedral beta 60 capsid at 3.3 A resolution (1988), J. Mol. Biol., 203, 1045-1070.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
detailed structure determination and analysis of enzyme complexed with heavy atoms, three-dimensional structure model	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23500	-	60 * 16000-16200 (beta) + 3 * 23500 (alpha), heavy riboflavin synthase, X-ray studies, analytical ultracentrifugation	Bacillus subtilis
100000	-	heavy riboflavin synthase	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine	Bacillus subtilis	-	riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	pure enzyme from mutant strain H94	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.027	-	purified enzyme	Bacillus subtilis
0.027	-	heavy enzyme	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine	-	Bacillus subtilis	riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Subunits

Subunits	Comment	Organism
polymer	60 * 16000-16200 (beta) + 3 * 23500 (alpha), heavy riboflavin synthase, X-ray studies, analytical ultracentrifugation	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
heavy riboflavin synthase	bifunctional enzyme complex that catalyzes formation of riboflavin from 5-amino-6-ribityl-amino-2,4-pyrimidinedione and 3,4-dihydroxy-2-butanone	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bacillus subtilis

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Release 2023.1 (January 2023)