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Literature summary for 2.5.1.9 extracted from

  • Harzer, G.; Rokos, H.; Otto, M.K.; Bacher, A.; Ghisla, S.
    Biosynthesis of riboflavin. 6,7-Dimethyl-8-ribityllumazine 5-phosphate is not a substrate for riboflavin synthase (1978), Biochim. Biophys. Acta, 540, 48-54.
    View publication on PubMed
Search for more literature for 2.5.1.9

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus subtilis
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 mutant WA 45
-
Bacillus subtilis
-
 heavy and light enzyme form
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0014
-
 partially purified enzyme Escherichia coli
0.027
-
 heavy enzyme Bacillus subtilis
0.5
-
 light enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Bacillus subtilis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
additional information no activity with lumazine 5'-phosphate Bacillus subtilis ?
-
 ?
additional information no activity with lumazine 5'-phosphate Escherichia coli ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bacillus subtilis
7
-
 assay at Escherichia coli