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Literature summary for 2.5.1.9 extracted from

  • Mitsuda, H.; Kawai, F.; Suzuki, Y.
    Assay methods, isolation procedures, and catalytic properties of riboflavin synthetase from spinach (1971), Methods Enzymol., 18B, 539-543.
No PubMed abstract available
Search for more literature for 2.5.1.9

Activating Compound

Activating Compound Comment Organism Structure
additional information assay carried out under reducing condition to avoid side reactions and because of the lability of the enzyme in presence of O2 Spinacia oleracea

General Stability

General Stability Organism
freezing, completely inactivates spinach enzyme Spinacia oleracea
high ionic strength protects yeast enzyme against inactivation Saccharomyces cerevisiae
reducing agents, e.g. cysteine, ascorbate or Na2SO4 stabilize yeast and spinach enzyme Saccharomyces cerevisiae
reducing agents, e.g. cysteine, ascorbate or Na2SO4 stabilize yeast and spinach enzyme Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
Avidin moderately, no prevention by biotin Saccharomyces cerevisiae
Cu2+
-
 Spinacia oleracea
Hg2+
-
 Spinacia oleracea
p-chloromercuribenzenesulfonate reversible by cysteine or 2-mercaptoethanol Saccharomyces cerevisiae
p-chloromercuribenzoate
-
 Saccharomyces cerevisiae
p-chloromercuribenzoate
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 6,7-dimethyl-8-(1'-D-ribityl)lumazine pH 7.0 Saccharomyces cerevisiae
0.045
-
 6,7-dimethyl-8-(1'-D-ribityl)lumazine pH 7.5 Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Spinacia oleracea
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Spinacia oleracea
-
 spinach
-

Oxidation Stability

Oxidation Stability Organism
lability in presence of O2 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae
additional information
-
 assay method Spinacia oleracea
0.00025
-
 partially purified enzyme Spinacia oleracea

Storage Stability

Storage Stability Organism
0-4°C, stable for 1 week in presence of saturated ammonium sulfate Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Spinacia oleracea riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Spinacia oleracea riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae
37
-
 assay at Spinacia oleracea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45
-
 Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 1 min, complete loss of activity Saccharomyces cerevisiae
80
-
 1 min, complete loss of activity Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Saccharomyces cerevisiae
7.5
-
-
 Spinacia oleracea