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Literature summary for 2.5.1.9 extracted from

  • Plaut, G.W.E.
    Studies on the nature of the enzymic conversion of 6,7-dimethyl-8-ribityllumazine to riboflavin (1963), J. Biol. Chem., 238, 2225-2243.
    View publication on PubMed
Search for more literature for 2.5.1.9

Activating Compound

Activating Compound Comment Organism Structure
2,3-dimercapto-1-propanol enhances activity Saccharomyces cerevisiae
2,3-dimercapto-1-propanol enhances activity Eremothecium gossypii
2-mercaptoethanol enhances activity Saccharomyces cerevisiae
2-mercaptoethanol enhances activity Eremothecium gossypii
N-acetylcysteine enhances activity Saccharomyces cerevisiae
N-acetylcysteine enhances activity Eremothecium gossypii
SO32- enhances activity Saccharomyces cerevisiae
SO32- enhances activity Eremothecium gossypii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 6,7-dimethyl-8-(1'-D-ribityl)lumazine
-
 Eremothecium gossypii
0.04
-
 6,7-dimethyl-8-(1'-D-ribityl)lumazine
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Eremothecium gossypii
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Eremothecium gossypii AG33
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Eremothecium gossypii
-
-
-
Eremothecium gossypii AG33
-
-
-
Escherichia coli
-
 strain ATCC 9637
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Saccharomyces cerevisiae
-
 Eremothecium gossypii

Reaction

Reaction Comment Organism Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Escherichia coli
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Saccharomyces cerevisiae
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Eremothecium gossypii
a

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Eremothecium gossypii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae
additional information
-
-
 Eremothecium gossypii
0.003
-
-
 Escherichia coli
0.052
-
 purified enzyme Saccharomyces cerevisiae
0.266
-
 purified enzyme Eremothecium gossypii

Storage Stability

Storage Stability Organism
-20°C, completely purified enzyme, stable for several months Saccharomyces cerevisiae
frozen, partially purified in dissolved ammonium sulfate precipitation pellet, stable several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Eremothecium gossypii riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Eremothecium gossypii AG33 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information overview Saccharomyces cerevisiae ?
-
 ?
additional information overview Eremothecium gossypii ?
-
 ?
additional information overview Eremothecium gossypii AG33 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Saccharomyces cerevisiae
37
-
 assay at Eremothecium gossypii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 2 cold lability, inclusion of 0.01 M sodium sulfite in the solution used in purification prevents inactivation Saccharomyces cerevisiae
92
-
 1 min, complete loss of activity Eremothecium gossypii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Eremothecium gossypii
7
-
-
 Escherichia coli
7
-
-
 Saccharomyces cerevisiae