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Literature summary for 2.5.1.89 extracted from

  • Hemmi, H.; Yamashita, S.; Shimoyama, T.; Nakayama, T.; Nishino, T.
    Cloning, expression, and characterization of cis-polyprenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus acidocaldarius (2001), J. Bacteriol., 183, 401-404.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activates Sulfolobus acidocaldarius

Cloned(Commentary)

Cloned (Comment) Organism
isolation of cis-polyprenyl diphosphate synthase, homology with known cis-polyprenyl diphosphate synthases, expression in Escherichia coli Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration: 0.5-2 mM, no activity with Mn2+ or Ca2+ Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q9HH76
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate about 35% of the activity with geranylgeranyl diphosphate Sulfolobus acidocaldarius 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + 7 isopentenyl diphosphate
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 Sulfolobus acidocaldarius 7 diphosphate + tritrans,heptacis-undecaprenyl diphosphate
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 ?
additional information dimethylallyl diphosphate and geranyl diphosphate scarcely react Sulfolobus acidocaldarius ?
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1 h, more than 90% of the activity is retained Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Sulfolobus acidocaldarius