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Literature summary for 2.5.1.87 extracted from
- A two-component enzyme complex is required for dolichol biosynthesis in tomato (2015), Plant J., 82, 903-914.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rer2, phylogenetic analysis, quantitative RT-PCR expression analysis, functional complementation of the yeast rer2DELTA dolichol mutant, production of dolichols in rer2DELTA strains expressing both SlCPT3 and SlCPTBP associating together in an enzyme complex, recombinant coexpression of C-terminally Myc-tagged SlCPT3 and FLAG-tagged SlCPTBP in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain LBA4404, recombinant expression of C-terminally Myc-tagged SlCPT3 in Escherichia coli | Solanum lycopersicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of RNAi-mediated knockdown of SlCPT3 expression in plants, SlCPT3 gene expression and also polyisoprenoid contents are reduced by approximately 60% and 40%, respectively. Although transgenic plantlets are recovered that exhibit a higher degree of SlCPT3 knockdown, they do snot survive to maturity. Interaction analysis in planta of SlCPT3 and SlCPTBP proteins by introducing C-terminally Myc-tagged SlCPT3 and FLAG-tagged SlCPTBP versions into Nicotiana benthamiana leaves in the endoplasmic reticulum lumeninal side | Solanum lycopersicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | luminal side | Solanum lycopersicum | 5789 | - |
| microsome | - |
Solanum lycopersicum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Solanum lycopersicum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | Solanum lycopersicum | - |
n diphosphate + ditrans,polycis-polyprenyl diphosphate | n = 10-55 | ? | |
| (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | Solanum lycopersicum MP-1 | - |
n diphosphate + ditrans,polycis-polyprenyl diphosphate | n = 10-55 | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | K7WCI9 | - |
- |
| Solanum lycopersicum MP-1 | K7WCI9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | - |
Solanum lycopersicum | n diphosphate + ditrans,polycis-polyprenyl diphosphate | n = 10-55 | ? | |
| (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | - |
Solanum lycopersicum MP-1 | n diphosphate + ditrans,polycis-polyprenyl diphosphate | n = 10-55 | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cis-prenyltransferase | - |
Solanum lycopersicum |
| SlCPT3 | - |
Solanum lycopersicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Solanum lycopersicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the class of enzymes known as cis-prenyltransferases (CPTs) elongate the trans-prenyl diphosphate intermediate with a cis-linked linear polymer of isopentenyl diphosphate units | Solanum lycopersicum |
| physiological function | the SlCPT3 and SlCPTBP proteins interact to form a functional dolichol synthase | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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