Cloned (Comment) | Organism |
---|---|
gene rer2, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strain KG219, expression of CaRER2 under control of the MET3 promoter in mutant strain JOS14 | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the PMET3RER2/rer2D mutant strain JOS18, and of ura3D::imm434/ura3D::imm434 RER2/rer2D::hisG mutant strain JOS14, and of ura3D::imm434/ura3D::imm434 RER2/rer2D::hisG-URA3-hisG mutant strain JOS13 | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Candida albicans | 5618 | - |
membrane | - |
Candida albicans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
- |
Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | Candida albicans | cis-prenyltransferase activity of the Candida albicans Rer2 homologue | n diphosphate + ditrans,polycis-polyprenyl diphosphate | n =10-55 | ? | |
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | Candida albicans ATCC MYA-2876 | cis-prenyltransferase activity of the Candida albicans Rer2 homologue | n diphosphate + ditrans,polycis-polyprenyl diphosphate | n =10-55 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q5AJZ3 | gene rer2 | - |
Candida albicans ATCC MYA-2876 | Q5AJZ3 | gene rer2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | cis-prenyltransferase activity of the Candida albicans Rer2 homologue | Candida albicans | n diphosphate + ditrans,polycis-polyprenyl diphosphate | n =10-55 | ? | |
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate | cis-prenyltransferase activity of the Candida albicans Rer2 homologue | Candida albicans ATCC MYA-2876 | n diphosphate + ditrans,polycis-polyprenyl diphosphate | n =10-55 | ? | |
additional information | dolichol synthesis using isopentenyl diphosphate | Candida albicans | ? | - |
? | |
additional information | dolichol synthesis using isopentenyl diphosphate | Candida albicans ATCC MYA-2876 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, about, sequence calculation | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
cis-prenyltransferase | - |
Candida albicans |
Rer2p | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Candida albicans |
General Information | Comment | Organism |
---|---|---|
malfunction | the morphological differentiation of a Candida albicans cis-prenyltransferase mutant is impaired | Candida albicans |
metabolism | cis-Prenyltransferase is the first enzyme of the mevalonate pathway committed to the biosynthesis of dolichol in eukaryotes | Candida albicans |
physiological function | cis-prenyltransferase Rer2 is required for protein glycosylation, cell wall integrity and hypha formation. Dolichols are essential not only for protein glycosylation and cell wall integrity but also for growth and development of Candida albicans | Candida albicans |