Crystallization (Comment) | Organism |
---|---|
on the basis of Escherichia coli undecaprenyl diphosphate synthase crystallographic structure the yeast Rer2p model is constructed. In the model three amino acid residues inserted into the sequence corresponding to the floor region of the tunnel extends the bottom loop what results in the required increase of the tunnel volume. Thermal fluctuations of this loop occasionally create a hole in the tunnel floor, making escape of polyprenol omega end out of the tunnel possible what switches off the control mechanism of product length thereby allowing a practically unlimited elongation process leading to an exponential distribution of longer chain polyprenols | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate | Saccharomyces cerevisiae | Rer2p Z-prenyltransferase synthesizes a well-defined family of polyprenols of 1318 isoprene residues with dominating C80 (16 isoprene residues) extending to C120. Two polyisoprenoid families with dominating C80 and C110 indicating the activity of both yeast Z-prenyltransferases Rer2p and Srt1p, respectively. The family of longer chain dolichols appearing in the stationary growth phase contains dolichols with even more than 50 isoprene units exhibiting monotonically decreasing amounts with increasing chain lengths. The family of shorter chain dolichols is characteristic of the Rer2p products synthesized during the logarithmic growth phase of yeast. It forms a well-defined family of dolichols with 1318 isoprene residues with a certain level of longer dolichols composed of up to 24 isoprene units | 13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 19 isopentenyl diphosphate | Saccharomyces cerevisiae | - |
19 diphosphate + di-trans,poly-cis-docosaprenyl diphosphate | dominating polyprenol with 22 isoprene residues, Srt1p products reaching C290 indicate the failure of a strict bacterial-like chain length control | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P35196 | - |
- |
Saccharomyces cerevisiae | Q03175 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate | Rer2p Z-prenyltransferase synthesizes a well-defined family of polyprenols of 1318 isoprene residues with dominating C80 (16 isoprene residues) extending to C120. Two polyisoprenoid families with dominating C80 and C110 indicating the activity of both yeast Z-prenyltransferases Rer2p and Srt1p, respectively. The family of longer chain dolichols appearing in the stationary growth phase contains dolichols with even more than 50 isoprene units exhibiting monotonically decreasing amounts with increasing chain lengths. The family of shorter chain dolichols is characteristic of the Rer2p products synthesized during the logarithmic growth phase of yeast. It forms a well-defined family of dolichols with 1318 isoprene residues with a certain level of longer dolichols composed of up to 24 isoprene units | Saccharomyces cerevisiae | 13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate | Rer2p Z-prenyltransferase synthesizes a well-defined family of polyprenols of 1318 isoprene residues with dominating C80 (16 isoprene residues) extending to C120 | Saccharomyces cerevisiae | 13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 19 isopentenyl diphosphate | - |
Saccharomyces cerevisiae | 19 diphosphate + di-trans,poly-cis-docosaprenyl diphosphate | dominating polyprenol with 22 isoprene residues, Srt1p products reaching C290 indicate the failure of a strict bacterial-like chain length control | ? |
Synonyms | Comment | Organism |
---|---|---|
Rer2p | - |
Saccharomyces cerevisiae |
Rer2p Z-prenyltransferase | - |
Saccharomyces cerevisiae |
Srt1p | - |
Saccharomyces cerevisiae |
Srt2p Z-prenyltransferase | - |
Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Saccharomyces cerevisiae | Rer2p is active in logarithmic phase, with dominating polyprenol with 16 isoprene residues | up |
Saccharomyces cerevisiae | Srt1p is expressed in stationary phase with dominating polyprenol with 22 isoprene residues | up |