Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000 (component A), + x * 60000 (component B), during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl diphosphate synthase activity when combined with each other, gel filtration | Micrococcus luteus |
60000 | - |
x * 20000 (component A), + x * 60000 (component B), during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl diphosphate synthase activity when combined with each other, gel filtration | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | Micrococcus luteus | the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | Micrococcus luteus B-P 26 | the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | O66127 and O66129 | O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase | - |
Micrococcus luteus B-P 26 | O66127 and O66129 | O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase | - |
Purification (Comment) | Organism |
---|---|
during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl pyrophosphate synthetase activity when combined with each other | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | Micrococcus luteus | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate | the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | Micrococcus luteus B-P 26 | 3 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | Micrococcus luteus | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 2 isopentenyl diphosphate | the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate | Micrococcus luteus B-P 26 | 2 diphosphate + all-trans-hexaprenyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000 (component A), + x * 60000 (component B), during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl diphosphate synthase activity when combined with each other, gel filtration | Micrococcus luteus |
Synonyms | Comment | Organism |
---|---|---|
hexaprenyl pyrophosphate synthetase | - |
Micrococcus luteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
component A is more stable against heat treatment than component B. The former retains 75% of its original activity for restoration after a 5 min treatment at 50°C while the latter loses 75% of its activity | Micrococcus luteus |