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Literature summary for 2.5.1.80 extracted from

  • Fan, A.; Chen, H.; Wu, R.; Xu, H.; Li, S.M.
    A new member of the DMATS superfamily from Aspergillus niger catalyzes prenylations of both tyrosine and tryptophan derivatives (2014), Appl. Microbiol. Biotechnol., 98, 10119-10129.
    View publication on PubMed
Search for more literature for 2.5.1.80

Cloned(Commentary)

Cloned (Comment) Organism
gene An13g01840, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Escherichia coli strain SoluBL21, subcloning in Escherichia coli strain XL1 BlueMRF' Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Aspergillus niger
Zn2+ slight inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 L-tryptophan pH 7.5, 37°C Aspergillus niger
0.71
-
 dimethylallyl diphosphate pH 7.5, 37°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Aspergillus niger
Mg2+ activates Aspergillus niger
additional information no significant effects on enzyme activity by K+, Na+, Mg2+, Mn2+, Co2+, or EDTA Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52700
-
-
 Aspergillus niger
53500
-
-
 Aspergillus niger
228000
-
 recombinant His6-tagged enzyme, gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A2R1N3 gene An13g01840 or ANI_1_660114
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to near homogeneity by nickel affinity chromatography and desalting gel filtration Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
 Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-4-methyl-DL-tryptophan
-
 ?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
 Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-5-methoxy-DL-tryptophan
-
 ?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
 Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan
-
 ?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
 Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan
-
 ?
dimethylallyl diphosphate + L-abrine
-
 Aspergillus niger diphosphate + ?
-
 ?
dimethylallyl diphosphate + L-beta-homotryptophan
-
 Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan
-
 ?
dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
additional information the enzyme catalyzes O-prenylation of L-tyrosine, EC 2.5.1.122, and also accepts 4-amino-L-phenylalanine for an N-prenylation and L-tryptophan for a C7-prenylation, EC 2.5.1.80. HRMS analysis of the enzyme product, overviews. 7-Prenylation substrate specificity, overview. No activity with D-tryptophan Aspergillus niger ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53500, recombinant His6-tagged enzyme, SDS-PAGE, x * 52700, about, sequence calculation Aspergillus niger

Synonyms

Synonyms Comment Organism
7-DMATS
-
 Aspergillus niger
ANI_1_660114
-
 Aspergillus niger
CAK41583
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0053
-
 L-tryptophan pH 7.5, 37°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aspergillus niger

General Information

General Information Comment Organism
evolution the enzyme is a member of the DMATS superfamily Aspergillus niger